冠心病关联标志物的初步研究  

The Preliminary Investigation on the Coronary Heart Disease-associated Biomarkers

作　　者：姜玫秀[1] 孙蕾[1] 王雪[1] 孙彬[1] 何国伟 李小菊[1] 

机构地区：[1]南开大学生命科学学院,天津300071 [2]天津泰达国际心血管医院,天津300457

出　　处：《南开大学学报（自然科学版）》2013年第1期28-33,共6页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：天津市自然基金(13JCYBJC24600;11JCYBJC10600);国家重点基础研究发展计划(2010CB945000);教育部科技发展中心博士点基金(20120031110020)

摘　　要：冠心病是心血管系统中的主要疾病之一,其关联标志物的研究对于冠心病的预防和治疗有着重要意义.分别选用来自于冠心病病人和正常人各20例血样,提取血样中单核细胞总蛋白,通过Western blot方法对部分在冠心病发生与发展中起重要作用的分子进行检测.结果显示,在冠心病病人血液中抗动脉粥样硬化分子ABCA1的表达量显著下降,而致动脉粥样硬化的分子CD36和PCSK9的表达量则明显上升,并且CD36在男性冠心病患者血液内的表达量高于女性患者.因而,CD36和PCSK9的过表达及ABCA1表达量的下降可能与中国人群冠心病的发生发展相关,有可能成为冠心病的关联标志物.Coronary heart disease （CHD） is one of the major diseases in cardiovascular system. It is very important to unveil the CHD-associated biomarkers for the prevention and treatment of this disease. In this study, twenty blood samples were collected from either healthy controls or patients with CHD followed by isolation of monocytes. The total cellular proteins were extracted from the monocytes and used to determine the protein expression of several molecules which have been documented to play an important role in the development of CHD by Western blot. Compared to controls. The results indicated that ABCA1 expression, an anti- atherogenic molecule, was significantly decreased while the expression of CD36 and PCSK9 （both are pro-atherogenic） was dramatically increased in CHD patient samples. In addition, CD36 expression was observed higher in male patients than that in female patients. Taken together, our findings indicate that the increased CD36 and PCSK9 expression and the decreased ABCA1 expression are associated with the development of CHD in Chinese population. Such changes may indicate that they can be considered as the CHD-associated biomarkers.

关 键 词：冠心病 ATP结合盒转运蛋白A1 前蛋白转化酶枯草溶菌素9 

分 类 号：R318[医药卫生—生物医学工程]