健康恒河猴外周血及肠系膜淋巴结中树突状细胞(Dendritic Cell)的亚群及分布特点  

Characterization of Dendritic Cells in the Peripheral Blood and Mesenteric Lymph Node of Normal Rhesus Macaques

作　　者：莎日娜[1] 孙明[2] 白纯[1] 袁玉华[3] 

机构地区：[1]天津医学高等专科学校,天津300222 [2]中国协和医科大学医学生物学研究所,北京100730 [3]天津市血液中心,天津300110

出　　处：《南开大学学报（自然科学版）》2013年第1期38-43,共6页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：天津市高等学校科技发展基金(20100607)

摘　　要：树突状细胞(Dendritic Cell,DC)作为天然免疫的重要组成部分,在抗原识别和抗原呈递过程中发挥重要作用.对DC细胞在恒河猴外周血及淋巴结组织中的细胞亚群及其分布频率进行初步观察.取健康源恒河猴外周血和肠系膜淋巴结,从中分离出淋巴细胞.利用流式细胞术检测分析不同亚群的DC细胞在其间的分布.结果表明,外周血及淋巴结中DC细胞比例均较低,但外周血中DC比例略高于肠系膜淋巴结.mDC亚群占总DC细胞数比例高于pDC细胞亚群,而肠系膜淋巴结中mDC比例相较外周血中有明显下降,统计学差异显著.测定了健康源恒河猴各亚群DC细胞在外周血和淋巴组织中的基础数值,为相关模型研究奠定了基础.Dendritic cells（DCs） are immune cells forming part of the mammalian immune system.Their main function is to process antigen material and present it on the surface to other cells of the immune system.Dendritic cells are important as messengers between the innate and adaptive immunity.Therefore,freshly isolated rhesus macaque DC from peripheral blood and mesenteric lymph nodes（mLN） were isolated and analysis different subsets distribution with multicolor flow cytometry.Our data show that the cell proportion of DC in the peripheral blood and mesenteric lymph nodes is low;meanwhile,the peripheral blood DC proportion is slightly higher than the mesenteric lymph nodes DC.In total,the mDC subsets of the total DC cell is higher than the pDC cell subsets,while the mDC proportion in mLN show significantly decrease compared to the peripheral blood.

关 键 词：恒河猴 树突状细胞 多色流式 pDC mDC 

分 类 号：Q78[生物学—分子生物学]