杀菌剂丙环唑的致癌性研究及其机制探讨  

The Carcinogenicity and the Mechanism Discussion of Propiconazole

作　　者：李双[1] 宋文华[1] 郭晶[1] 孙瑞娟[1] 宋千千 

机构地区：[1]天津工业大学环境与化学工程学院,天津300387 [2]Linden Hall School For Girls,Pennsylvania 17543,U.S.A

出　　处：《南开大学学报（自然科学版）》2013年第1期89-93,共5页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：国家自然科学基金(21007045)

摘　　要：研究了丙环唑及其代谢物对小鼠胚胎细胞的恶性转化作用.结果发现丙环唑及其代谢活化产物均能够引起小鼠胚胎成纤维细胞发生转化,并形成大量典型的Ⅲ型转化灶.进一步利用刀豆球凝集实验和琼脂集落实验探究转化细胞表面刀豆球蛋白A(ConA)受体分布和锚定非依赖生长特性,确定丙环唑诱导细胞恶性转化的能力,发现转化细胞在ConA稀释液中凝集成块;在半固体琼脂培养液中成集落生长,失去接触抑制的特性,具有成瘤的可能,因此丙环唑对人类健康的潜在威胁不容忽视.In order to investigate and research the propiconazole carcinogenicity,the mouse embryonic cell morphological transformation test was performed.The propiconazole dosage ranged from 0,40,60 to 80 μg/mL were designed.The results showed that propiconazole and its metabolites activation products can induce NIH/3T3 cell malignant transformation,and a large number of typical type Ⅲ transformed focuses had been formed.The ConA agglutination test and soft agar assay has been carried out to explore the acceptor of concanavalin A（ConA） on the surface of the transformed cell distributed and the non-dependent growth characteristics,determine the malignant transformation ability of the propiconazole induced cell,and prove the transformed cell has been agglutinated in the ConA solution.And also found that it is grown in a colony in the semi-solid agar culture medium,and lost the contact inhibition properties,which is possible to form the tumor,and therefore the potential threat of propiconazole to the human health can not be ignored.

关 键 词：丙环唑 纳米氧化锌 恶性转化 刀豆球蛋白凝集实验 琼脂集落实验 

分 类 号：X592[环境科学与工程—环境工程]