Synthesis and Solid Phase Extraction Performance Study of NNAL-specific Molecularly Imprinted Polymers Using Dummy Templates  

NNAL模拟模板分子印迹聚合物的合成与固相萃取性能

作　　者：张志丹[1] 郑赛晶[2] 杨俊[1] 王维妙[2] 刘百战[2] 朱晓兰[1] 

机构地区：[1]中国科学技术大学化学系,合肥230026 [2]中国烟草总公司卷烟烟气重点实验室,上海200082

出　　处：《Chinese Journal of Chemical Physics》2013年第3期361-368,I0004,共9页化学物理学报（英文）

摘　　要：Dummy molecularly imprinted polymers （DMIPs） for 4-（methylnitrosamino）-1-（3-pyridyl）-1-butanol （NNAL） were produced using three structural analogues as dummy template molecules. The chosen analogues were 4-（acetymethylamino）-1-（3-pyridyl）-butanol, 4- （methylamino）-1-（3-pyridyl）-1-butanol, and 1-（3-pyridyl）-1,4,-butanediol. The molecular recognition characteristics of the produced polymers were evaluated by X-ray photoelec- tron spectroscopy （XPS） and Fourier transform infrared spectroscopy （FT-IR）. Interactions between NNAL and methacrylic acid should be cooperative hydrogen bonds while the ni- trogen atom of the pyridine ring and the oxygen atom of the nitroso group in NNAL are two of the hydrogen-bond acceptors. It was further demonstrated that DMIP synthesized by 4-（acetymethylamino）-1-（3-pyridyl）-butanol had the best binding performance by XPS and FT-IR. Then dummy molecularly imprinted solid phase extraction （DMISPE） was developed for the determination of the analyte using the hit polymer as the sorbing material. Under optimal conditions, the recovery of NNAL dissolved in standard solution reached 93%. And the investigated polymer exhibited much higher binding of NNAL when nicotine was acted as the competitive molecule. Also the proposed method was applied to the measurement of NNAL spiked in blank urine samples with recoveries ranging from 87.2% to 101.2%.

关 键 词：Molecularly imprinted polymer Dummy template 4-（Methylnitrosamino）-1-（3-pyridyl）-1-butanol Solid phase extraction 

分 类 号：O6[理学—化学]