水稻核糖核酸酶T2蛋白质在叶片生长和盐胁迫过程中的表达研究  

Expression Profiling of Rice RNase T2 Proteins in Leaves and Seedlings under Salt-stressed Conditions

作　　者：曾祥然[1] 魏健[1] 贾霖[1] 关明俐[1] 刘钊[1] 兰金苹[1] 刘丽娟[1] 李莉云[1] 刘国振[1] 

机构地区：[1]河北农业大学生命科学学院,河北保定071001

出　　处：《核农学报》2013年第6期743-749,共7页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金(31171528)

摘　　要：核糖核酸酶(Ribonucleases,RNase,RNS)T2普遍存在于各种生物中,其保守性提示了功能的重要性。在水稻基因组中有8个RNase T2成员,本试验采用基于抗体的蛋白质组学策略,用免疫印迹(Western Blotting,WB)技术检测了它们在叶片生长及盐胁迫条件下的表达,发现OsRNS1-OsRNS7蛋白质在叶片中有表达,其中OsRNS4主要在苗期表达,提示其在苗期发挥作用,其它蛋白质主要在成株期表达,但没有检测到OsRNS8的表达;水稻OsRNS4在盐胁迫条件下表达上调,提示其可能在盐胁迫应答过程中发挥作用,其它RNase T2蛋白质的表达大多随盐胁迫时间的延长而下降。将RNase T2蛋白质的表达与转录信号进行比较,发现二者在部分基因中有一定的相关性。本试验获得的数据为揭示水稻RNase T2基因的功能提供了线索。Ribonucleases（RNase,RNS）T2 are found in almost all kinds of organisms,the conservation suggested the importance of their functions.Eight RNase T2 members are present in the rice（Oryza sativa L.） genome.Using antibody-based proteomics strategy,the expression patterns of the RNase T2 proteins in leaves and seedlings under salt-stressed conditions were surveyed by Western blotting（WB）.The results indicated that the OsRNS1-OsRNS7 proteins were expressed in leaves,while the expression of OsRNS8 was not detected.Interestingly,the OsRNS4 protein was mainly expressed in seedling stage,revealing that it played a role in seedling stage.The other proteins were expressed in adult stages.The expression of OsRNS4 protein was up-regulated under salt-stressed condition,suggesting that it plays a role in the response to salt-stress,while the expressions of most of the other RNase T2 proteins were down-regulated under the stressed condition.Comparison between the protein expression abundance and transcriptional intensity revealed that certain correlations existed in some genes.Taken together,the data obtained in this experiment provides interesting clues for uncovering the function of rice RNase T2 genes.

关 键 词：水稻 核糖核酸酶(RNase)蛋白质 免疫印迹 基于抗体的蛋白质组学 

分 类 号：S511[农业科学—作物学]