水稻CMS-WA和CMS-Y育性恢复基因Rf3和Rf4的等位性分化  

Allelic Differentiations of the Rf3 and Rf4 Genes on Fertility Restoration in Rice with Wild Abortive and Y Type Cytoplasmic Male Sterility

作　　者：蔡健[1,2] 兰伟[1] 廖秋平[2] 马同富[1] 

机构地区：[1]阜阳师范学院生命科学学院,安徽阜阳236041 [2]华南农业大学植物分子育种重点实验室,广东广州510642

出　　处：《核农学报》2013年第6期750-757,共8页Journal of Nuclear Agricultural Sciences

基　　金：国家“863”计划(2006AA100101-2)

摘　　要：为了检测水稻第1染色体上恢复基因Rf3和第10染色体上恢复基因Rf4的等位性分化,利用8个携带Rf3基因座位,16个携带Rf4基因座位的染色体单片段代换系(chromosome single segmentsubstitution line,SSSL)和华粳籼74(HJX74)为父本,野败型(WA)细胞质雄性不育系珍汕97A(ZsA)和Y型(Y)细胞质雄性不育系Y华农A(HnA)为母本杂交,采用分子标记辅助选择的方法,鉴定携带基因型Rf3rf3/Rf4rf4的F1杂种株并对其花粉和小穗育性进行考察。结果表明:(1)24个SSSLs和HJX74对于2个不育系的恢复力存在着显著的不同,携带Rf3基因座位的SSSLs恢复力均低于携带Rf4基因座位的SSSLs,并且低于对照品种HJX74;SSSL S6对于WA-CMS的花粉小穗育性分别为7.2%和15.5%,对于Y-CMS的花粉小穗育性分别为1.3%和12.4%,表现出最弱的恢复力;SSSLs S14-S18和SSSL S20对这2种不育系的平均花粉和小穗育性分别高于70%和85%,表现出较强的恢复力。(2)在恢复基因Rf3和Rf4基因座分别鉴定出3个和4个等位基因,由弱到强依次命名为Rf3-1、Rf3-2、Rf3-3和Rf4-1、Rf4-2、Rf4-3、、Rf4-4,受体亲本HJX74的基因型为Rf3Rf3/Rf4Rf4,其Rf3的恢复性强于Rf4恢复性。(3)在HJX74的遗传背景下,WA型不育细胞质的可恢复性好于Y型不育细胞质。To detect the naturally occurring allelic variation at the fertility restorer（Rf） loci on chromosomes 1（Rf3） and 10（Rf4）,eight SSSLs carrying Rf3 locus,sixteen SSSLs possessing Rf4 locus and HJX74 were crossed to two GMS lines（A-lines）,such as Zhenshan97A（ZsA,WA） and Y-HuanongA（HnA,Y）,respectively,the F1 plants,carrying the genotype Rf3rf3/Rf4rf4,were selected by marker-assisted selection,and their phenotype for pollen and spikelet fertility were evaluated.The results were as follows.（1） There were much differences in restoring abilities among the twenty-four SSSLs and HJX74.The restoration abilities of SSSLs carrying Rf3 locus were weaker than that of SSSLs with Rf4 locus and HJX74.SSSL S6 carrying Rf3 locus exhibited 7.2%（15.5%） and 1.3%（12.4%） pollen（spikelet） fertility of F1 plants and possessed the weakest restoring ability to WA-CMS and Y-CMS.Out of sixteen SSSLs with Rf4 locus,high levels of pollen fertility（70%） and spikelet fertility（85%） were observed in the crosses of A-lines/SSSLs S14-S18 and SSSL S20,which showed stronger restorer ability to WA-CMS and Y-CMS.（2） Based on the pollen and seed fertility of the F1 hybrids,the Rf3 and Rf4 genes were classified respectively into four alleles,namely Rf3-1,Rf3-2,Rf3-3 and Rf3-4 for Rf3,and Rf4-1,Rf4-2,Rf4-3 and Rf4-4 for Rf4.HJX74 carried the genotype Rf3Rf3/Rf4Rf4and showed that the effect of Rf3 was larger than that of Rf4.（3） In inheritance background of HJX74,WA-CMS was restored more easily than Y-CMS.

关 键 词：水稻 单片段代换系 质核互作雄性不育系 恢复力 

分 类 号：S511[农业科学—作物学]