1,25(OH)_2D_3和维甲酸对巨核白血病细胞的增殖抑制作用  

Inhibition of Proliferation of Megakaryoblastic Leukemic Cells by 1,25-Dihydroxyvitamin D_3 and Retinoic Acid

作　　者：程涛[1] 严舫[1] 万景华[2] 殷建林[2] 

机构地区：[1]长海医院血液科,200433 [2]中国医学科学院血液学研究所,天津300020

出　　处：《第二军医大学学报》1991年第1期24-27,共4页Academic Journal of Second Military Medical University

摘　　要：本文报道了两种生理性诱导剂——活性维生素D_3[1,25(OH)_2D_3,简称D_3]和维甲酸(RA)对原始巨核白血病细胞的增殖抑制作用。采用活细胞计数、[~3H]-TdR和[~3H]-UR参入以及克隆形成率测定3项指标,对一株新建的人原始巨核白血病细胞系(HIMeg)进行实验,结果表明:10^(-9)～10^(-6)mol/LD_3和RA对巨核白血病细胞的生长抑制作用呈明显量效与时效关系,10^(-7) mol/L D_3可使其克隆形成率减少90％以上。从而进一步说明了诱导分化剂对白血病及D_3对骨髓纤维化的治疗机制,提示D_3和RA可能有新的治疗用途。The effect of inhibition of proliferation of a novel human megakaryoblastic leukemia cell line (HIMeg) by two physiological factors, 1.25-dihydroxyvitamin D3 [1,25(OH)2 D3] and 13-cis-retinoic acid (RA), was investigated. At the range of 10-9 - 10-6 mol/L, 1,25(OH)2 D3 and RA showed significant inhibition of proliferation of the megakaryoblastic leukemia cells,which was demonstrated by count of survival cells,incorporation of [3H]-TdR and [3H]-UR, and cloning efficiency. From above, it can further explain the m.echanism of differentiation-inducers and the effect of 1,25(OH)2D3 on myelofibrosis. It is possible for 1,25(OH)2D3 and RA to be used for a new -treatment.

关 键 词：维生素D3 维甲酸 白血病 增殖抑制 

分 类 号：R965[医药卫生—药理学]