乳化剂OP增敏DDTC光度法测定大豆中微量铜(Ⅱ)  

Determination of trace copper (Ⅱ) in Soybean by DDTC spectrophotometric with co-enhanced sensitivity of OP

作　　者：王少玲[1] 丘秀珍[1] 彭翠红[1] 

机构地区：[1]韶关学院化学与环境工程学院,广东韶关512005

出　　处：《韶关学院学报》2013年第4期39-42,共4页Journal of Shaoguan University

摘　　要：利用乳化剂聚乙二醇辛基苯基醚（乳化剂OP）胶束对二乙基二硫代氨基甲酸钠（DDTC）与铜（Ⅱ）显色反应增溶、增敏作用,建立了分光光度法测定水相体系中微量铜（Ⅱ）的新方法.研究表明,在pH=10的介质中,铜（Ⅱ）与DDTC及乳化剂OP形成的络合物,λmax=454 nm,摩尔吸光系数ε=1.2×104,铜含量在0~2.8μg/mL范围内线性关系良好（R=0.999 7）.用该法测得大豆中的铜平均质量浓度为0.902μg/g,加标回收率为99.50%~100.80%,RSD为0.37%（n=6）,结果令人满意.The paper uses the enhanced sensitivity effect of OP on the color reaction of DDTC and Cu （ I1 ）, and the spectrophotometry method to determine trace copper in water. The results showed that Cu （lI） reacted with DDTC and OP to form a green blue complex in the buffer solution at pH 10.0. The maximum absorption wavelength was 454 nm.The apparent molar absorptivity was 1.2×10^4. The absorbance showed good linearity with Cu （ Ⅱ ） in the range of 0-2.8 μg/mL, and the correlation coefficient was 0.999 7.The proposed method has been applied to the determination of Cu in Soybean samples. The average content of Cu in Soybean was 0.902 μg/g with the recovery of 99.50%-100.80%, and the relative standard deviation of 0.37 （n=6）. The results were satisfactory.

关 键 词：乳化剂OP 二乙基二硫代氨基甲酸钠(DDTC) 铜 大豆 

分 类 号：O657.32[理学—分析化学]