三叶海棠叶中水溶性总糖含量的测定  

Determination of the water-soluble total sugars in Malussieboldii

作　　者：肖美凤[1] 吴萍[1] 陈慧[1] 刘芳[1] 文礼章[2] 

机构地区：[1]湖南中医药大学药学院中药现代化重点实验室,湖南长沙410208 [2]湖南农业大学生物安全科学技术学院,湖南长沙410128

出　　处：《湖南中医药大学学报》2013年第5期62-64,共3页Journal of Hunan University of Chinese Medicine

基　　金：湖南中医药科技局资助项目(201161);湖南省科学技术厅科研条件创新专项一般项目(2012TT2012);湖南省中药学重点学科资助(湘教发[2011]76号)

摘　　要：目的测定三叶海棠叶中的水溶性总糖含量。方法三叶海棠叶的水提液经酸水解-乙酸乙酯萃取后,采用试样空白的蒽酮-硫酸比色法,以葡萄糖为对照品进行总糖含量测定。结果葡萄糖在7～35μg/mL范围内,吸光度与浓度的线性关系良好(r=0.998 1,n=6),平均回收率为94.1%,RSD=1.0%(n=6);3批三叶海棠叶中的水溶性总糖平均含量为13.1%。结论利用酸水解-乙酸乙酯萃取的差示光谱法(△A法)消除了试样基体有色和脂溶性物质的干扰,使显色后的吸收峰明显改善,结果可靠,重复性好;为三叶海棠叶中水溶性总糖的含量测定提供了一种可行的方法。Objective To determine the water-soluble total sugars in water extract from Malussieboldii.Methods The total sugars in water extract of Malussieboldii after acid hydrolysis and extracted with ethyl acetate were determined with the anthrone-sulfuric colorimetry in which the glucose was used as standard reference.Results The concentration of glucose showed a good linearity with absorbance in the range of 7～35 μg/mL at 620 nm（r=0.998 1,n=6）.The average water soluble total sugars of three shipments of Malussieboldii was 13.1%,the average recovery was 94.1%,and RSD was 1.0 %（n=6）.Conclusion The method can improve the absorption peak remarkably with differential spectrophotometry（△A method） to eliminate the colored matrix and organic interference,which can provide a reliable and repeatable method for determination of the water-soluble total sugars in Malussieboldii extractions.

关 键 词：三叶海棠叶 水溶性总糖 蒽酮-硫酸比色法 差示光谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]