长白和蓝塘猪印记基因IGF2和H19的时空表达研究  

A Study of the mRNA Level of IGF2 and H19 in Landrace and Lantang Swine

作　　者：吴嫦丽[1,2] 许继国[1] 吴同山 卫恒习[1] 李莉[1] 李紫聪[1] 吴珍芳[1] 张守全[1] 

机构地区：[1]华南农业大学动物科学学院,广东省农业动物基因组学与分子育种重点实验室,广东广州510642 [2]广东医学院基础医学院,广东湛江524023 [3]东莞市畜牧科学研究所,广东东莞523086

出　　处：《华南农业大学学报》2013年第3期388-393,共6页Journal of South China Agricultural University

基　　金：国家科技支撑计划(2011BAD19B03);国家973计划(2011CB944202;2010CB945001)

摘　　要：运用实时荧光定量PCR技术,检测IGF2和H19印记基因在长白猪和蓝塘猪中的表达水平.测定了1日龄和180日龄2个阶段的基因在各组织中的转录表达水平,按照生长性状分组,对组间的表达差异进行了比较分析.分析不同日龄长白、蓝塘猪各组织中IGF2、H19的表达量.结果显示,仔猪初生体质量大的组肝脏组织中IGF2基因表达水平显著高于初生体质量轻的组;180日龄猪皮脂厚的组脂肪组织的IGF2基因表达量高于皮脂薄的组;1日龄仔猪肝脏、肌肉和胃组织中IGF2表达量高于其他测定组织和180日龄的各组织;180日龄蓝塘猪肾脏组织中的H19表达量显著升高.In this study,the mRNA expression levels of imprinted gene IGF2 and H19 in Landrace and Lantang swine were investigated through real-time fluorescence quantitative PCR.The pigs were grouped according to growth traits.The relationships between mRNA expression levels with growth and reproductive traits were analyzed.The results showed the IGF2 mRNA expression levels of liver in the heavy birth body mass group was higher than that of the light birth body mass group.When 180 days old,the IGF2 mRNA expression levels of fat in the thick sebum group was higher than that of the thin sebum group.The IGF2 mRNA expression levels of liver,muscle and stomach in 1 day old piglet were higher than those in other tissues.The H19 mRNA expression levels of kidney in 180 days old Landrace pig increased significantly.

关 键 词：猪 IGF2基因 H19基因 基因表达水平 生长性状 RT-PCR 

分 类 号：Q756[生物学—分子生物学]