合成肉桂酰胺类化合物对芒果炭疽病菌的抑菌活性及其构效关系分析  

Antifungal activity and structure-function relationships of synthetic cinnamic amide compounds against Colletotrichum gloeosporioides

作　　者：李一方[1,2] 万树青[1] 

机构地区：[1]华南农业大学天然农药与化学生物学教育部重点实验室,广东广州510642 [2]怀集县农业技术推广中心,广东肇庆526400

出　　处：《仲恺农业工程学院学报》2013年第2期16-21,共6页Journal of Zhongkai University of Agriculture and Engineering

基　　金：广东省自然科学基金(990703;010319);天然农药与化学生物学教育部重点实验室基金(2005)资助项目

摘　　要：采用菌丝生长速率法,测定了以黄皮酰胺(N-甲基-N-顺-苯乙烯-肉桂酰胺)为模板人工合成的20种肉桂酰胺类化合物对芒果炭疽病菌(Colletotrichum gloeosporioides)的离体抑菌活性,并对合成肉桂酰胺类化合物取代基与其抑菌活性之间的关系进行了分析.结果表明,(E)-3-(3,4-二甲基)-N-苯乙基-3-(吡啶-4)丙烯酰胺(化合物1)有较好的抑菌效果,对芒果炭疽病菌的EC50为67.95μg/mL;吡啶环上的-Cl对该类化合物的抑菌活性没有显著的提高作用;酰胺基团碳端β位碳上连接的苯环上的各种取代基对其抑菌活性的影响也不明显;酰胺基团N端γ位碳上连接的苯环上的取代基中,羟基能促进病菌生长;酰胺上同时连接吡啶环和苯基不能显著提高其抑菌活性,其抑菌活性不及单一连接苯基.Antifungal activity against CoUetotrichum gloeosporioides of 20 kinds of different cinnamic am- ide compounds, which were synthesized based on the construction of Lansiumamide B （ N-methyl-N-cis- styryl-cinnamamide） , were examined by the evaluation growth rate. The relationship between different substituent and the antifungal activity in vitro of cinnamic amide compounds were also analyzed and com- pared. The result indicated that the （E）-3-（3,4-dimethylphenyl）-N-phenethyl-3-（pyridin-4-yl） acry- lamide （ the compound 1 ） had good antifungal effect. The EC50 of compound 1 against C. gloeosporioides was 67.95 μg/mL, and the -Cl in the pyridine had no significant effect on the activity. The ring of benzene connected with B-carbon on the carbon side of amide groups also had no significant effect on the activity. The activity was not increased significantly by the pyridine ring and phenyl connect at the same carbon side of amide, and the higher antifungal activity of one connected phenyl lonely was achieved.

关 键 词：肉桂酰胺类化合物 黄皮酰胺 芒果炭疽病菌(Colletotrichum gloeosporioides) 构效关系 

分 类 号：S436.67[农业科学—农业昆虫与害虫防治]