不同土壤水分对丹参叶片总酚酸类成分积累及相关酶活性的影响  

Effect of Different Soil Moistures on Total Phenolic Acid Composition Accumulation and Relevant Enzymatic Activity of Salvia Miltiorrhiza Lamina

作　　者：梁宗锁[1,2] 杨东风[1] 杨宗岐[1] 韩蕊莲[1,2] 刘晓蕾[2] 

机构地区：[1]浙江理工大学生命学院,杭州310018 [2]西北农林科技大学生命科学院,陕西杨凌712100

出　　处：《浙江理工大学学报（自然科学版）》2013年第4期573-578,共6页Journal of Zhejiang Sci-Tech University(Natural Sciences)

基　　金：浙江理工大学科研启动基金(1204806-Y;1204822-Y);高等学校博士学科点专项科研基金(20110204110028)

摘　　要：以丹参(Salvia miltiorrhiza Bunge)为材料,采用盆栽实验,研究丹参叶片在不同土壤水分(田间持水量的35%、55%、75%)条件下总酚酸类成分积累及相关酶活性的变化规律。结果表明干旱胁迫后期,丹参叶片总酚酸类成分积累明显提高,表现为总酚酸含量以55%处理最高,35%处理次之,75%处理最低,其中55%处理总酚酸含量达到75%处理的1.50倍,35%处理达到75%处理的1.31倍。处理前期,55%土壤含水量能够促进PAL酶活性提高,而处理中后期55%和35%土壤含水量均能够促进TAT酶活性提高,但干旱胁迫对C4H酶活性影响不显著。丹参叶片总酚酸类成分的积累与PAL和C4H酶相关,未发现与TAT有明显关系。这为丹参叶片资源的开发和丹参规范化种植与灌溉提供了理论参考,也为丹参药材的质量控制提供了指导。This paper studies total phenolic acid composition accumulation and change rules of relevant enzymatic activity of salvia miltiorrhiza lamina under the condition of different soil moistures（35%, 55% and 75% of field capacity） through pot experiment with Salvia miltiorrhiza Bunge as the material. The result shows that, in the later period of drought stress, the total phenolic acid composition accumulation of salvia miltiorrhiza lamina significantly increases. The total phenolic acid content is the highest when treated at 55%, followed by 35% and 75%. The total phenolic acid content treated at 55% is 1.50 times of that at 75%; that at 35% is 1.31 times of that at 75%. In the earlier stage of treatment, 55% soil moisture can promote the increase of PAL enzymatic activity, while 55 % and 35 % soil moisture in the later period of treatment can promote the increase of TAT enzymatic activity, but drought stress doesn＇t have significant influence on C4H enzymatic activity. The accumulation of total phenolic acid composition of salvia miltiorrhiza lamina is related to PAL and C4H enzyme and its obvious relation with TAT is not found. This provides theoretical reference for the development of salvia miltiorrhiza lamina resources and standard plantation and irrigation of salvia miltiorrhiza and provides guidance for the quality control of salvia miltior- rhiza medicinal material.

关 键 词：土壤水分 总酚酸 丹参 酶活性 

分 类 号：R282.2[医药卫生—中药学]