水稻端粒酶RNA模板基因序列鉴定体系的建立  

Establishment of Identification System of Telomerase RNA Template Gene Sequence in Rice

作　　者：杨力媛[1] 郑洁[1] 马登旭[1] 马国兴[1] 刘小川[1] 

机构地区：[1]浙江理工大学生物工程研究所,杭州310018

出　　处：《浙江理工大学学报（自然科学版）》2013年第4期590-594,共5页Journal of Zhejiang Sci-Tech University(Natural Sciences)

摘　　要：利用水稻端粒酶对端粒序列的合成作用,对模板基因序列进行定点突变,构建了pCambia1301-T突变型、pCambia1301-Y野生型载体。经农杆菌介导,选用水稻不同品种和组织,探索候选序列的鉴定体系。结果显示,不同水稻品种诱导愈伤,差异显著,其中中花11号可快速诱导愈伤,比广陆矮4诱导时间缩短一半,用成熟胚和幼胚分别诱导愈伤,无明显差异;对突变型、野生型、正常组织进行基因组DNA端粒鉴定和端粒酶活性分析,发现突变型端粒酶活性明显高于对照。这些均表明该鉴定体系完整、可靠,为端粒酶RNA模板基因序列的鉴定建立了有效的方法。This paper conducts site-directed mutagenesis for template gene sequence by using the combining effect of rice telomerase for telomere sequence and establishes pCambia1301-T mutant type and pCambial301-Y wild type carrier; selects different rice varieties and tissues through agrobacterium mediation and explores the identification system of candidate sequence. The result shows that different rice varieties have significance differences in callus inductiom No. 11 Middle Flower can rapidly induce callus and the induction time is half of that of GLA 4;mature embryo and young embryo have no significant difference in callus induction; the analysis on identification of genome DNA telomerase activity of telomere for mutant type, wild type and normal tissues finds that mutant type telomerase activity is significantly higher than the control group. All this shows that this identification system is complete and feasible and establishes an effective method for the identification of RNA template gene sequence of telomerase.

关 键 词：水稻 端粒酶RNA 模板序列鉴定技术 定点突变 

分 类 号：Q785[生物学—分子生物学]