人血型糖蛋白A衍生物对恶性疟原虫裂殖子识别及入侵红细胞的效应  

Effects of Derivatives of Human Glycophorin A on the Recognition and Invasion of Plasmodium falic parum into Erythrocytes

作　　者：王向东[1] 骆训懿[1] 李建新[1] 魏尧梅[1] 

机构地区：[1]第二军医大学训练部生物化学教研室

出　　处：《第二军医大学学报》1991年第4期306-309,共4页Academic Journal of Second Military Medical University

摘　　要：用电泳纯人血型糖蛋白A(GPA)制得下列衍生物:(1)用胰蛋白酶酶解GPA分离得GPA糖肽;(2)制备GPA和GPA糖肽两种抗体;(3)制备去糖GPA(dGPA);(4)用人红细胞膜全脂分别重组成含GPA及dGPA的两种脂质体。用上述制品对FCC-1/HN株恶性疟原虫裂殖子实验,发现:(1)GPA脂质体可与裂殖子结合,而dGPA脂质体呈阴性反应。(2)GPA,GPA糖肽,GPA抗体,GPA糖肽抗体及GPA脂质体均有阻止恶性疟原虫裂殖子入侵人红细胞的效应。Electrophoretic-purified human glycophorin A (GPA) was used to produce its derivatives: (1) glycopeptides were separated and purified from GPA by trypsin digestion; (2) preparation of GPA-antibody and GPA glycopeptide-antibody; (3) preparation of deglycosylated GPA (dGPA); (4) incorporating GPA or dGPA into human RBC membrane lipids to form two kinds of liposomes. The products described above were used to test Plasmodium falciparum FCC1/HN merozoites for their ability to invade human erythrocytes. It was found that GPA-liposomes were able to bind with merozoites and dGPA-liposomes had a negative reaction. GPA, GPA glycopeptide, GPA-antibody, GPA glycopeptide-antibody and GPA-liposome all had the effect to hinder the invasion of merozoites into human erythrocyte while dGPA-liposome had no such an effect.

关 键 词：疟原虫裂殖子 红细胞 人血型糖蛋白 

分 类 号：R392.12[医药卫生—免疫学]