彭泽鲫养殖池塘不同生境微生物基因组DNA的提取  

Different Habitats in the Breeding Pond Pengze Crucian Carp Microbial Genomic DNA Extraction

作　　者：郭建军[1] 傅筱冲[1] 龚小华[1] 付锦楠[1] 何顺华[1] 

机构地区：[1]江西省科学院微生物研究所,江西南昌330029

出　　处：《江西科学》2013年第3期344-346,383,共4页Jiangxi Science

基　　金：江西省科学院国家级预研项目(2009-1-06)

摘　　要：比较3种DNA提取方法(溶菌酶法、CTAB法及珠磨法)对养殖池塘几种生境(底泥、饲料、彭泽鲫肠道内容物及肠道壁)菌群PCR-DGGE分析的影响。结果表明,以3种提取方法获得的DNA为模板均能进行16SrDNA V3区特异性片段扩增,但不同DNA提取方法对池塘不同生境菌群DGGE指纹图谱存在显著影响;人工养殖的彭泽鲫肠道内容物菌群与肠道壁的菌群存在一定的相似性,肠道内容物菌群比肠道壁菌群丰富。This study compareed the effects of three different DNA extraction methods（lysozyme digestion,CTAB method and beadmill） on analyzing bacteria from different micro-ecological environments in an farming pond（pond sludge,feed,intestinal content and intestinal wallofgrass carp） by PCR-DGGE.The results showed that 16S rDNA V3 fragments were successfully amplified from samples extracted by three DNA extraction methods in allmicro-ecological environments,but different DNA extraction methods had remarkable influence on DGGE fingerprints of bacteria under different micro-ecological environments.The results showed that the bacterial community displayed a certain similarity between intestinal contents and intestinal wall of the fish under artificial rearing conditions.The bacterial community of intestinal contents was richer than that of intestinal wall.

关 键 词：彭泽鲫(Pengze crucian carp) 养殖池塘 菌群 PCR-DGGE 

分 类 号：Q933[生物学—微生物学]