MicroRNA生物合成相关基因在肝癌中的表达及其意义  

Expression and significance of microRNA biosynthesis related genes in hepatocellular carcinoma

作　　者：闫兆伟[1] 朱海涛 董琼珠[1] 周闯[2] 张晓飞[2] 任宁[2] 叶青海[2] 钦伦秀[2] 

机构地区：[1]复旦大学生物医学研究院,200032 [2]复旦大学附属中山医院肝肿瘤外科,200032

出　　处：《国际消化病杂志》2013年第3期193-196,211,共5页International Journal of Digestive Diseases

摘　　要：目的通过检测肝癌和癌旁组织中microRNA(miRNA)合成相关基因的mRNA表达,分析其与肝癌恶性行为的关系。方法运用实时荧光定量聚合酶链反应(RT-PCR)技术检测肝癌组织及其配对癌旁组织中miRNA生物合成相关基因的mRNA表达水平,比较分析其表达水平差异及其与肝癌预后的关系。结果 RT-PCR结果显示,与相应的癌旁组织相比,肝癌组织中细胞核内miRNA生物合成相关基因Drosha、DGCR8、DBR1、ADAR、Exportin 5的mRNA表达水平显著升高(P<0.0001),而细胞质内miRNA生物合成相关基因Dicer、AGO2、GEMIN3、GEMIN4的mRNA表达水平显著下降(P<0.0001)。肝癌组织中DBR1和Exportin 5 mRNA高表达患者的总生存率高于低表达患者(P<0.05),癌旁组织中DGCR8mRNA高表达患者的总生存率高于低表达患者(P<0.05)。结论 Dicer的表达可能在肝癌发生发展过程中起重要作用。DBR1、Exportin 5和DGCR8可以作为肝癌治疗的潜在干预靶点。Objective To investigate the relationship between the expression of microRNA（miRNA） biosynthesis related genes and the malignant phenotype of hepatocellular carcinoma （HCC）. Methods The expression levels and prognostic significance of miRNA biosynthesis related genes mRNA in HCC with paried adjacent nontumorous tissues were assessed by real-time polymerase chain reaction （RT-PCR）. Results The results of RT-PCR showed that the expressions of Drosha, DGCR8, DBR1, ADAR, Exportin 5 in HCC tissues were up-regulated compared with adjacent tissues （P〈0. 0001）, and the expression of Dicer, AGO2, GEMIN3, GEMIN4 in HCC tissues were down-regulated compared with adjacent tissues （P〈0. 0001）. The overall survival rate of patients with high DBR1 and Exportin 5 expression was higher than patients with low expression in HCC tissues （P〈0. 05）. The overall survival rate of patients with high DGCR8 expression was higher than patients with low expression in adjacent tissues （P〈0.05）. Conclusion Dicer expression may play an important role in the development of HCC. DBR1, Exportin 5 and DGCR8 can serve as a potential intervention target for HCC treatment.

关 键 词：肝细胞癌 MICRORNA DROSHA DGCR8 EXPORTIN 5 DICER AGO2 GEMIN3 GEMIN4 DBR1 ADAR 

分 类 号：R735.7[医药卫生—肿瘤]