p38 MAPK通路在血晶素诱导肺泡巨噬细胞发挥抗炎功能中的作用  

Role of p38 MAPK Signal Pathway Involved in Anti-Inflammatory Mechanism of Hemin in Rat Primary Alveolus Macrophages

作　　者：宁杰[1] 王莉[1] 李艳华[1] 庞庆丰 

机构地区：[1]云南省第一人民医院麻醉科,云南昆明650032 [2]无锡医学院病理生理学教研室,江苏无锡214122

出　　处：《昆明理工大学学报（自然科学版）》2013年第3期80-84,共5页Journal of Kunming University of Science and Technology(Natural Science)

基　　金：国家自然科学基金资助项目(81270126)

摘　　要：研究血晶素诱导肺泡巨噬细胞发挥抗炎作用的机制及其信号通路,为临床应用血晶素打下理论基础.采用在体支气管肺泡灌洗获取大鼠肺泡巨噬细胞(AMs),在AMs培养液中分别加入内毒素、血晶素组、SB203580和锌原卟啉.各组细胞刺激30 min后,检测AMs中P-p38MAPK的表达水平;刺激24 h后,检测AMs中血红素加氧酶-1(HO-1)的表达水平,同时测量细胞精氨酸酶活性、吞噬能力和培养上清中一氧化氮含量.结果发现,血晶素可以引起AM细胞中P-p38MAPK表达明显增多(P<0.05),血晶素刺激24 h后HO-1表达水平和精氨酸酶活性明显增强(P<0.05),一氧化氮分泌减少.SB203580和锌原卟啉则可阻断上述变化.上述结果表明,血晶素诱导AMs发挥抗炎功能通过激活p38MAPK途径和增加HO-1表达水平实现.The possible anti - inflammatory mechanism and signal pathway of hemin in rat primary alveolus macrophages （AMs） are investigated in this paper. AMs are isolated from the brochoalveolar lavage fluid in si- tu and stimulated by LPS, Hemin, SB203580 or Znpp. After AMs are stimulated for 30 mins, protein was ex- tracted from the collected cells for measuring P- p38MAPK by Western blot. After AMs are stimulated for 24 hours, the supernatants are collected for measuring NO; AMs are collected for measuring arginase activity and phagocytes ability; and protein is extracted from the collected cells for measuring HO - 1 by Western blot. The results show that Hemin can effectively increase the level of HO - 1 and P - p38MAPK（ P 〈 0.05 ） , up - regu- late arginase aetivity（P 〈0.05） , and decrease NO production（P 〈 0.05 ）. SB203580 or Znpp can abolish these above effects. These results suggest that HO - 1 is easily induced in AMs by hemin. HO - 1 induction in rat AMs has higher arginase activity, phagocytose ability and lower NO production. These changes may be p38MAPK dependent.

关 键 词：肺泡巨噬细胞 血红素加氧酶-1 炎症 P38丝裂原活化蛋白激酶 

分 类 号：R3[医药卫生—基础医学]