天山雪莲PsaH基因的克隆及序列分析  

Cloning and Sequence Analysis of PsaH Gene from Sasussured involucrata Kar. et Kir

作　　者：张林华[1] 邱红林[1] 刘超[1] 王爱英[2] 邓福军[3] 祝建波[2] 

机构地区：[1]石河子大学生命科学学院,石河子832003 [2]石河子大学农业生物技术重点实验室,石河子832003 [3]新疆农垦科学院,石河子832000

出　　处：《生物技术通报》2013年第6期75-79,共5页Biotechnology Bulletin

基　　金：国家自然基金资助项目(31160049)

摘　　要：Ps I H(Subunit H of photosystem I)是属于光合系统I亚族的分子量约10 kD的内在膜蛋白,是真核生物特有的3个组成光合系统I的亚基之一,在植物光合作用中起重要作用。从天山雪莲(Sasussured involucrataKar.et Kir)叶片全长cDNA文库中,筛选克隆得到PsaH基因。序列分析表明,该基因的开放阅读框为432 bp,编码143个氨基酸,包括由47个氨基酸组成的转运肽和96个氨基酸组成的成熟PS I H。预测该蛋白质相对分子量为15.04 kD,理论等电点(Theoretical pI)为9.81,存在一个跨膜结构。序列比对表明,SikPsaH编码蛋白与蓖麻(Ricinus communis)、毛果杨(Populus trichocarpa)PsaH基因编码的蛋白质具有83.3%的相似性。系统进化树显示,天山雪莲与石蒜(Lycoris radiata)亲缘关系最近。。Ps I H is an intrinsic membrane protein of 10 kD that is a subunit of photosystem I（Ps I）,Ps I H is one of the three PSI subunits found only in eukaryotes,plays an important role in plant photosynthesis.A cDNA clone encoding a PS I H subunit of photosystem I was cloned from Sasussured involucrata Kar.et Kir leaf cDNA library.Sequence analysis indicated that the psaH cDNA contained an open reading frame of 432 nucleotides encoding a 143 amino acid which consisted of a 47 amino acid transit peptide and a 96 amino acid mature PS I H peptide.Forecasted the protein relative molecular weight is 15.04 kD,Theoretical pI was 9.81,contains a span membrane structure.Sequence alignment indicated that the deduced amino acid sequence of Sasussured involucrata Kar.et Kir showed high identity of 83.3% to those of PsaH from Ricinus communis and Populus trichocarpa.Phylogenetic analysis further showed that the SikPsaH gene has the nearest relationship with Lycoris radiate.

关 键 词：天山雪莲 PsaH基因 克隆 序列分析 

分 类 号：S567.239[农业科学—中草药栽培] Q943.2[农业科学—作物学]