防腹泻布拉酵母培养条件的响应面法优化  

Optimization of Cultivation Conditions of Saccharomyces boulardii Against Diarrhea Using Response Surface Method

作　　者：胡小媛[1,2] 滕达[2] 张勇[2] 毛若雨[2] 王秀敏[2] 黄建忠[1] 王建华[2] 

机构地区：[1]福建师范大学生命科学学院工业微生物教育部工程研究中心,福州350108 [2]中国农业科学院饲料研究所基因工程室农业部饲料生物技术重点开放实验室,北京100081

出　　处：《生物技术通报》2013年第6期194-199,共6页Biotechnology Bulletin

基　　金：科技支撑计划课题(2013BAD10B02,2011BAD26B02);中国农业科学院基本科研业务费(1610032012042)

摘　　要：在摇瓶水平上对布拉酵母(Saccharomyces boulardii)培养基及培养条件进行优化。先通过单因子法筛选最优碳源、氮源及无机盐,再利用响应面法优化发酵培养基组分及培养条件,采用Plackett-Burman法筛选影响布拉酵母生长的主要因素,用最陡爬坡试验及Box-Benhnken设计进一步优化选定的主要因素。得出最佳发酵培养基为:麦芽糖2%,蛋白胨2%,酵母浸提取物2.08%,培养基初始pH值为6;最佳发酵条件为:温度29.6℃,转速250 r/min,250 mL三角瓶培养基装量29 mL,接种量为5%,培养时间为24 h。优化后的布拉酵母菌体湿重达到32.2 g/L,比优化前(20.9 g/L)提高了54.1%;优化后活菌数达8.3×108CFU/mL,比优化前(5.5×108CFU/mL)提高了50.9%。The fermentation medium and conditions of Saccharomyces boulardii were optimized based on single-factor experiments and response surface method.Single-factor experiments were performed to select the optimal carbon source,nitrogen source and inorganic salt.Response surface method was used to optimize the fermentation medium and conditions.A Plackett-Burman design was used to evaluate the effects of eight factors;the path of steepest ascent and the Box-Behnken design were performed for further optimization.The optimal fermentation medium contained 2% of maltose,2.08% of yeast extract,2% of glucose,with initial pH value of 6.0.The optimal fermentation conditions were as follows ：5% inoculums added to 250 mL erlenmeyer flask with 29 mL medium volume were cultured with shaking speed 250 r/min at 29.6℃ for 24 h.In the optimal fermentation medium and conditions,the cell wet weight of Saccharomyces boulardii increased from 20.9 g/L to 32.2 g/L,increased by 54.1%,the number of viable yeast increased from 5.5×108CFU/mL to 8.3 ×108CFU/mL,increased by 50.9%.

关 键 词：布拉酵母 响应面法 优化 培养条件 防腹泻 

分 类 号：S816.3[农业科学—饲料科学]