益智仁不同极性提取物抗老年痴呆作用的研究  

Study on Anti-senile Dementia Function of Different Polar Extracts of Alpinia oxyphylla

作　　者：石绍淮[1] 张晨宁[1] 刘冰[1] 刘芷[1] 毕开顺[2] 贾英[1] 

机构地区：[1]沈阳药科大学中药学院,沈阳110016 [2]沈阳药科大学药学院,沈阳110016

出　　处：《中国药房》2013年第27期2507-2510,共4页China Pharmacy

基　　金：辽宁省科学技术计划立项课题(No.2011412004-1);辽宁省教育厅高等学校科研项目(No.2009T097)

摘　　要：目的:筛选益智仁抗老年痴呆的活性部位,探讨益智仁不同极性提取物对老年痴呆模型小鼠学习记忆能力的影响。方法:采用不同极性的溶剂分别萃取益智仁提取物。将108只雄性小鼠随机均分为空白(等容生理盐水)、模型(等容生理盐水)、石杉碱甲(0.61g/kg)与益智仁石油醚(11.1g/kg)、氯仿(11.1g/kg)、乙酸乙酯(11.1g/kg)、正丁醇(11.1g/kg)、水提物(11.1g/kg)、总提物(11.1g/kg)组,灌胃给药,每天1次,连续21d。给药第16天腹腔注射氢溴酸东莨菪碱(1mg/kg),连续5d;给药第16天开始进行Morris水迷宫实验,连续6d。行为学测试结束后,采集小鼠血清、海马和脑皮质标本,测定其血清超氧化物歧化酶(SOD)、大脑皮质乙酰胆碱转移酶(ChAT)和海马乙酰胆碱酯酶(AchE)活力。结果:与模型组比较,水迷宫定位航行实验中正丁醇组小鼠第3～5天单日逃避潜伏期与这3天平均逃避潜伏期显著缩短(P<0.01或P<0.05);空间探索实验中正丁醇组小鼠目标象限的游泳时间显著延长(P<0.01);正丁醇组小鼠SOD和ChAT活性显著增强,AchE活性显著减弱(P<0.01)。结论:正丁醇部位是益智仁抗老年痴呆的活性部位,其机制可能与抗氧化、增加ChAT活性和抑制AchE活性以改善胆碱能系统有关。OBJECTIVE： To screen the effective fraction of Alpinia oxyphylla, and to explore the effects of effective extracts of A. oxyphylla on learning and memory ability in mice with learning and memory required-disorder induced by scopolamine. METHODS： Different extracts were extracted by different polar solvents. 108 male mice were randomly divided into blank group （constant volume of normal saline）, model group （constant volume of normal saline）, huperzine A group （0.61 g/kg）, vaether petrolei extract of A. oxyphylla group （11.1 g/kg）, chloroform extract of A. oxyphylla group （11.1 g/kg）, acetic ether extract of A. oxyphylla a group （11.1 g/kg）, aqueous extract of A. oxyphylla group （11.1 g/kg）,water extract of A. oxyphylla group, total extract of A. oxyphylla group（11.1 g/kg）. They were given medicine intragastrically once a day for consecutive 21 days.Mouse were injected？into？abdominal？cavity scopolamine hydrobromide （1 mg/kg） for consecutive 6 days since 16 day of treatment.Morris water maze test was carried out for consecutive 6 days since 16 day of treatment. At the end of praxiology test, the serum, hippocampus and pallium samples were collected. The activities of SOD, ChAT and AchE in serum were determined. RESULTS： Compared with model group, the latency time of n-butyl alcohol group on 3rd-5th day and average latency time of 3 days can be significantly shortened in water maze navigation experiment（P〈0.01 or P〈0.05）; the swimming time of n-butyl alcohol group can be significantly increased in target quadrant in space exploration experiment （P〈0.01）. The activities of SOD and ChAT were increased significantly in n-butyl alcohol group, while the activities of AchE were decreased（P〈0.01）. CONCLUSIONS： The n-butyl alcohol fraction of A. oxyphylla is the effective fraction on senile dementia, and its mechanism may be closely related to antioxidant effect and the improvement of cholinergic system by increasing ChAT activity and inhibiting AchE acti

关 键 词：益智仁 抗老年痴呆 学习记忆 活性部位 正丁醇 

分 类 号：R965[医药卫生—药理学] R285[医药卫生—药学]