正交试验优选南山茶花中总黄酮的提取工艺  

Optimization of Extraction Technology of Total Flavonoids from the Flowers of Camellia semiserrata by Orthogonal Test

作　　者：尚志春[1] 王宝昌[1] 张微微[1] 彭倩[1] 唐玲[1] 王永奇[1] 史丽颖[1] 

机构地区：[1]大连大学药物研究所,辽宁大连116622

出　　处：《中国药房》2013年第27期2526-2528,共3页China Pharmacy

基　　金：国家自然科学基金资助项目(No.30772714)

摘　　要：目的:优选南山茶花中总黄酮的提取工艺。方法:采用乙醇-水提取法从南山茶花中提取总黄酮,在单因素试验基础上,选取乙醇体积分数、提取时间、提取温度、料液比为考察因素,以总黄酮收率为评价指标,采用正交试验优选工艺。结果:总黄酮的最佳提取工艺为用20倍量的85%乙醇,95℃下提取2次,每次3h。在此条件下,总黄酮收率为1.52%。结论:优选的提取工艺合理、可行,可用于南山茶花中总黄酮的提取。OBJECTIVE： To optimize the extraction technology for total flavonoids from the flowers of Camellia semiserrata. METHODS： The total flavonoids were extracted from the flowers with EtOH-H2O. The extraction technology was optimized by orthogonal test with enthanol concentration, extraction time, extraction temperature and ratio of material to liquid as factor using yield of total flavonoids as index. RESULTS： The optimal extraction technology was as follows： 20-fold 85% ethanol, extracting 2 times at 95 ℃ for 3 hours. By this technology, the yield of total flavonoids was 1.52%. CONCLUSIONS：Optimized technology was reasonable and feasible,which can be applied for the extraction of total flavonoids from the flowers of C. semiserrata.

关 键 词：南山茶花 总黄酮 提取工艺 正交试验 

分 类 号：R284.2[医药卫生—中药学] R283[医药卫生—中医学]