五倍子的HPLC指纹图谱研究  

Study on HPLC Fingerprint of Galla Chinensis

作　　者：郭小瑞 李里[2] 孙哲[3] 徐美玲 马福敏 郭乃菲 

机构地区：[1]中医药大学,辽宁大连116600 [2]沈阳军区总医院,沈阳110016 [3]北京中医药大学,北京100029

出　　处：《中国药房》2013年第27期2546-2548,共3页China Pharmacy

基　　金：辽宁省科学技术计划(博士启动基金计划)项目(No.20111130)

摘　　要：目的:建立五倍子药材的高效液相色谱指纹图谱。方法:色谱柱为Kromasil C18(250mm×4.6mm,5μm),流动相为1%冰醋酸水溶液-甲醇(梯度洗脱),柱温为25℃,检测波长为280nm,流速为1.0ml/min。应用相似度评价软件和SPSS13.0软件对不同来源的14批药材进行相似度评价和系统聚类分析。结果:以没食子酸峰为内参比峰,共标定了五倍子药材指纹图谱中14个共有峰。14批样品中有12批的相似度在0.900以上,为推荐品;有2批小于0.900,为非推荐品。聚类分析结果与此相同。结论:该方法灵敏度高、重复性好、专属性强,可用于五倍子药材的质量评价。OBJECTIVE： To establish the HPLC fingerprint of Galla Chinensis. METHODS： The separation was performed on Kromasil C18（250 mm×4.6 mm, 5 μm） column, with mobile phase consisted of 1% glacial acetic acid-methanol （gradient elution） at the flow rate of 1.0 ml/min. The detection wavelength was set 280 nm, and column temperature was 25 ℃. The systematic cluster analysis of 14 batches of samples was conducted with SPSS13.0 software; the similarity of them was evaluated using fingerprint similarity evaluation system. RESULTS： 14 common peaks were identified using gallic acid as internal reference peak; among 14 batches, the similarity of 12 batches were more than 0.900 as recommended products; other 2 batches were lower than 0.900 as non-recommended products. It was same to the results of cluster analysis. CONCLUSIONS： Established method is sensitive, reproducible and specific, and can be used for the quality evaluation for Galla Chinensis.

关 键 词：五倍子 指纹图谱 高效液相色谱法 系统聚类分析 

分 类 号：R284.1[医药卫生—中药学] R917[医药卫生—中医学]