血凝反应法检测黄毛鼠对抗凝血杀鼠剂抗性的可行性研究  

Study on the feasibility of blood clotting response test for determining resistance to anticoagulant rodenticide in Rattus losea

作　　者：隋晶晶[1] 高志祥[1] 姚丹丹[1] 冯志勇[1] 颜世祥[1] 

机构地区：[1]广东省农业科学院植物保护研究所,广东省植物保护新技术重点实验室,广东广州510640

出　　处：《中国媒介生物学及控制杂志》2013年第3期208-210,214,共4页Chinese Journal of Vector Biology and Control

基　　金："十二五"国家科技支撑计划(2012BAD19B02);广东省自然科学基金(S2011010000895);广东省科技计划项目(2011B031500001)~~

摘　　要：目的探讨血凝反应法检测黄毛鼠对抗凝血杀鼠剂抗性的可行性。方法以杀鼠灵(warfarin)抗药性区分剂量(10mg/kg)单次灌胃处理结合致死期食毒抗性检测法(LFP)筛选出黄毛鼠敏感种群和抗性种群,在不同时间段采集试鼠血浆,通过检测试鼠血浆的凝血酶活度(PCA)建立黄毛鼠凝血反应标准曲线,并分析抗性区分剂量处理后抗性个体与敏感个体PCA的变化差异。结果建立了黄毛鼠的凝血反应标准曲线:INR(y)=34.984/x+0.688(x=PCA)(R2=0.992);以10mg/kg为区分剂量单次灌胃处理后,抗药性黄毛鼠个体的PCA虽有所下降,但可在2～3d内恢复到正常凝血水平的17%左右;敏感个体的PCA可下降到很低,且不能恢复。结论证实了以血凝反应法检测黄毛鼠对抗凝血剂抗药性的可行性:杀鼠灵10mg/kg为区分剂量单次灌胃处理4d后,以PCA=16.5(或INR=4.4)作为阈值来区分黄毛鼠抗药性与敏感性个体,是准确、简便的抗药性判定方法。Objective To investigate the feasibility of blood clotting response （BCR） test for determining the resistance to anticoagulant rodenticide in Rattus losea. Methods Sensitive and resistant populations of R. losea were screened out by a single gavage of warfarin at a discriminating dose of 10 mg/kg and lethal feeding period test. Plasma was collected from test rats at different time points; the standard curve of BCR was established by measuring the percentage clotting activity （PCA） of plasma, and the difference in PCA between resistant and sensitive individuals treated with a discriminating dose of warfarin was analyzed. Results The standard curve of BCR in R. losea was established as follows： INR（y）=34.984/x＋0.688（x=PCA）（R2 =0.992）. After the single gavage of warfarin at a discriminating dose of 10 mg/kg, the PCA of resistant individuals decreased, but it was restored to 17% of normal level within 2-3 d; the PCA of sensitive individuals decreased dramatically and was not restored. Conclusion This study confirms the feasibility of BCR test for determining the resistance to anticoagulant rodenticide in R. losea. It is accurate and simple to differentiate resistant and sensitive individuals of R. losea by a single gavage of warfarin （10 mg/kg） and using PCA=16.5 （or INR=4.4） as the threshold 4d later.

关 键 词：黄毛鼠 杀鼠灵 抗性检测 血凝反应 

分 类 号：S443[农业科学—农业昆虫与害虫防治] S481.4[农业科学—植物保护]