心肌内钙细胞化学及EDX能谱分析  

Calcium Cytocheiuical Localization and EDX Microanalysis in Myocardial Cells

作　　者：杜晓冰 杨勇骥[2] 王根英[2] 郑尊[2] 

机构地区：[1]长征医院病理科,200003 [2]第二军医大学训练部中心实验室,200433

出　　处：《第二军医大学学报》1991年第6期523-526,共4页Academic Journal of Second Military Medical University

摘　　要：用焦锑酸钾法对心肌内钙进行细胞化学定位,经X射线能量失散谱分析(EDX)法显示,OPP法及PPP法对细胞内结合钙均有特异性反应,OPP法更为敏感。钙细胞化学证明心肌内钙的分布是高度隔室化的,其主要钙贮存池是肌浆网和线粒体,其中肌浆网含钙量更高,焦锑酸钾钙细胞化学定位及EDX能谱分析对进一步探讨钙在心肌细胞代谢及损伤过程中的作用机制有重要意义。Intracellular calcium distribution was studied in myocardial cells of mice by using pyroantimonate cytochemistry and EDX microanalysis. The results demonstrated that both OPP and PPP methods had a selective localization of intracellular bound calcium, and OPP method was more sensitive to calcium. The distribution of calcium in myocardial cells is highly departmentalized, and its mam pools were confined to sarcoplasmic reticulum and mitochondria. Calcium eylochemical localization is very useful in studying the effect of calcium in metabolism and pathogenesis of myocandial cells.

关 键 词：心肌细胞 钙细胞化学 X射线 

分 类 号：R542.2[医药卫生—心血管疾病]