硝普钠对特发性血小板减少性紫癜模型小鼠脾脏还原型谷胱甘肽、氧化型谷胱甘肽含量的影响机制研究  

Study on the influence of sodium nitroprusside on idiopathic thrombocytopenia purpura model mouse spleen antioxidants glutathione and oxidized glutathione content

作　　者：金呈强[1] 贾彦霞[2] 董海新[1] 周建伟[1] 赵元明[1] 胡小行[1] 刘仿[3] 

机构地区：[1]济宁医学院附属医院检验科,272029 [2]济宁市第一人民医院,272000 [3]广东医学院微生物与免疫学教研室,524023

出　　处：《国际免疫学杂志》2013年第4期314-318,共5页International Journal of Immunology

基　　金：山东省自然科学基金（ZR2010HL038）;济宁市科技发展计划资助项目（2012jnje16）

摘　　要：目的探讨一氧化氮（NO）供体药物硝普钠腹腔注射特发性血小板减少性紫癜（ITP）模型小鼠后，对不同时间点小鼠脾脏内还原型谷胱甘肽（GSH）和氧化型谷胱甘肽（GSSG）的影响及意义。方法ITP模型小鼠分成NO供体药物硝普钠（SNP）组和磷酸盐缓冲液（PBS）对照组，SNP组用相同浓度（1mg／ml）的NO供体药硝普钠稀释液进行腹腔注射，PBS对照组使用相同体积的磷酸盐缓冲液进行腹腔注射，用药第5min、15min、25min、35min和45min后，检测外周血中NO及一氧化氮合酶（NOS）的表达，酶联免疫法（ELISA）法测定脾脏中GSH和GSSG含量。结果SNP组小鼠血清中NO及NOS在第5min，15min，25min，35min和45rain的浓度均高于PBS对照组（NO：t5=6．24，t15=8．65，t25=9．87，t35=11．20，t45=13．07；NOS：t5=5．47，t15=6．55，t25=8．04，t35=9．28，t45：11．54，P均〈0．05），相邻测量时间点NO及NOS含量均具有统计学意义（NO：t5-15=3．04，t15-25=4．11，t25-35=3．82，t35-45=3．54；NOS：t5-15=3．98，t15彩=5．04，t25-35=5．12，t35-45=4．26，P均〈0．05），GSH浓度随着时间的延长而降低，相邻时间点测量值有统计学意义（t5-15=5．62，t15-25，u25=4．90，t25-35，=5．21，t35-45=5．07，P均〈0．05），GSSG的含量随着时间的延长而升高，相邻时间点测量值有着显著性差异（t5-15=4．07，t15-25=6．57，t25-35=4．98，t35-45=5．41，P均〈0．05），GSH浓度分别与NO、NOS呈负相关（r=0．625，r=-0．534，均P〈0．01），GSSG浓度分别与NO、NOS呈正相关（r=0．714，r=0．687，均P〈0．01）。结论在ITP小鼠模型体内，NO供体药物硝普钠以时间依赖的形式减少GSH的含量，硝普钠可通过影响抗氧化系统GSH／GSSG来发挥相应的生理功能。Objective To investigate the impact of sodium nitroprusside （SNP） on the concentration of antioxidants glutathione（GSH） and oxidized glutathione （GSSG） in the spleens of idiopathic thrombocytope- nia purpura （ITP）. Method ITP model mice were divided into the nitric oxide （NO） donor drug SNP groups and phosphate buffered saline （PBS） control group. Intraperitoneal injection with the concentration （ 1 mg/ml） of SNP for SNP group while intraperitoneal injection using the same volume of PBS for PBS control group. The expression of NO, nitric oxide synthase （NOS） in peripheral blood and GSH / GSSG content in spleen was de- tected after medication for 5 min, 15 min ,25 min,35 min and 45min. Results Serum concentration of NO and NOS in SNP group were higher than the PBS control group in the first 5 rain, 15 min ,25 min ,35 min and 45min （ NO ： t5 = 6. 24,t15 = 8.65, t25 = 9. 87, t35 = 11.20, t45 = 13.07 ; NOS ： t5 = 5.47, t15 = 6. 55, t25 = 8. 04, t35 = 9. 28,t45 = 11. 54, all P 〈 0.05 ）. NO and NOS eontent had statistical difference in each measurement time point （ NO ： t5-15 = 3.04,t15-25 = 4. 11, t25-35 = 3.82, t35-45 = 3.54 ; NOS ： t5-15 = 3.98, t15-25 = 5. 04, t25-45 = 5. 12, t35-5 = 4. 26, all P 〈 0.05 ）. GSH concentration reduced with time while GSSG content increased with time （ GSH ： t5-15 = 5.62, t15-25 = 4. 90, t25-35 = 5.21, t35-45 = 5. 07 ; GSSG ： t5-15 = 4. 07, t15-25 = 6. 57, t25-35 = 4. 98, t35-45 = 5.41 ,all P 〈 0.05 ）. GSH concentrations were negatively correlated with NO, NOS （r =-0. 625, r =-0. 534, both P 〈0.01 ）. GSSG concentrations were positively correlated with NO, NOS （ r = 0. 714, r = 0. 687, both P 〈 0.01 ）. Conclusion NO donor drug SNP reducd the level of GSH in a time-dependent pattern in ITP mice, SNP may play a physiological function by affecting the system of antioxidant GSH / GSSG.

关 键 词：一氧化氮 一氧化氮合酶 谷胱甘肽 特发性血小板减少性紫癜 

分 类 号：R965[医药卫生—药理学]