人参二醇对鱼藤酮、MPP+诱导的PC12细胞损伤的作用  

Effects of panoxadiol on PC12 cell injury induced by rotenone or MPP

作　　者：金晓蓉[1] 孙馨[2] 林筱洁[2] 苗青[2] 高瑞兰[2] 方桂伦[1] 

机构地区：[1]义乌市中心医院内科,322000 [2]浙江省中医院血液病研究所

出　　处：《浙江医学》2013年第12期1143-1146,共4页Zhejiang Medical Journal

基　　金：浙江省卫生厅中医药科技研究项目基金资助(2008CA115)

摘　　要：目的观察人参二醇对鱼藤酮、MPP+诱导的大鼠嗜铬细胞瘤损伤的影响。方法以大鼠嗜铬细胞瘤PC12细胞为研究对象,以鱼藤酮(0.3、1、3、10、30μmol/L)或MPP+(0.1、0.3、1、3mmol/L)诱导细胞损伤。设阴性对照组、人参二醇对照组(10、25、50、75、100mg/L)、鱼藤酮(3μmol/L,24h)或MPP+(1mmol/L,48h)组,人参二醇(10、25、50、75、100mg/L)联合鱼藤酮(3μmol/L)或MPP+(1mmol/L)组。采用MTT法检测细胞增殖活性;PI和Hoechst 33342染色检测细胞坏死和凋亡;并以免疫组织化学测定细胞酪氨酸羟化酶(TH)的表达。结果人参二醇(10、25、50、75、100mg/L)本身不会抑制PC12细胞增殖,其中50、75mg/L人参二醇还可促进细胞增殖;各浓度人参二醇对鱼藤酮诱导的细胞损伤均不具有保护作用;在MPP+处理诱导细胞损伤后,50、75mg/L人参二醇可提高细胞增殖活性,但人参二醇对细胞凋亡和坏死及TH的表达无影响。结论人参二醇(50、75mg/L)对MPP+诱导的PC12细胞损伤有保护作用,其作用机制与促进细胞增殖有关;人参二醇对鱼藤酮诱导的细胞损伤无明显保护作用。Objective To evaluate the effects of panoxadiol on rat pheochromocytoma PC12 cell injury induced by rotenone or MPP＋. Methods PC12 cell injury was induced by rotenone （0.3, 1, 3, 10, 30 IJ mol/L,24h） or MPP＋ （0.1, 0.3, 1, 3 mmol/L, 48h）. Panoxadiol （10, 25, 50, 75,100 mg/L） was used to treat rotenone or MPP＋ induced PC12 cells. Cell proliferation was assessed by MTT method, cell necrosis and apoptosis was detected by flow cytometry with PI （propidium iodide） and Hoechst 33342 stain, respectively. The expression of tyrosine hydroxylase was measuared via immunohistostaining. Results Panoxadiol （10, 25, 50, 75, lOOmg/L） had no affcts on proliferation of PC12 cells and had no protection effects on cell injury induced by rotenone. When PC 12 cell injury was induced by MPP＋, panoxadiol（50,75 mg/L） promoted cell proliferation but had no effects on necrocytosis and apoptosis and the expression of tyrosine hydroxylase. Conclusion Panoxadiol （50,75 mg/L） might have pro- tection effects on PC12 cell injury induced by MPP＋, which may be related to facilitation of cell proliferation; however, panoxadiol has no protection effects on cell injury induced by rotenone.

关 键 词：人参二醇 鱼藤酮 MPP+ 大鼠 PC12细胞 

分 类 号：R285.5[医药卫生—中药学]