马来酰亚胺聚谷氨酸天冬氨酸聚合物偶联性能研究  

Coupling Characterizations of Maleimide-functionalized Aspartylated Polyglutamic Acid

作　　者：耿旭[1] 卢光莉[2] 黄静[3] 

机构地区：[1]河南大学医学院,河南开封475004 [2]河南大学工商管理学院豫商研究中心,河南开封475004 [3]华东师范大学生命科学学院,上海200062

出　　处：《河南师范大学学报（自然科学版）》2013年第3期117-119,共3页Journal of Henan Normal University(Natural Science Edition)

基　　金：国家自然科学基金(31170920)

摘　　要：马来酰亚胺聚谷氨酸天冬氨酸(Mal-PGA-Asp)是在天然高分子聚谷氨酸(PGA)基础上化学修饰而获得,能够偶联含巯基的多肽;成为新型药物载体.波长分析和双键鉴定反应显示Mal-PGA-Asp带有马来酰亚胺基团;电泳检测表明Mal-PGA-Asp可以有效偶联P40(还原型多肽)及mGLP-1(TCEP还原氧化型多肽);这为Mal-PGA-Asp药物载体广泛应用奠定了基础.Maleimide-functionalized aspartylated polyglutamic acid（Mal-PGA-Asp） as a novel drug delivery carrier that was obtained by the chemical modification polyglutamic acid（PGA） as a natural polymer could conjugate to peptides with thiol.The wavelength scanning Mal-PGA-Asp and the color fading reaction of it by potassium permanganate indicated that Mal-PGA-Asp contained the maleimide group;electrophoresis analysis showed that the Mal-PGA-Asp could efficiently conjugate to P40（the reduced form） or mGLP-1（the oxidized form） reduced by TCEP.The study laid the foundation that Mal-PGA-Asp as drug delivery carrier will get widely application.

关 键 词：聚谷氨酸 马来酰亚胺 药物载体 电泳 

分 类 号：Q71[生物学—分子生物学]