二-(2,4-二氯苯甲酰异羟肟酸)二苯基合锡大鼠血浆蛋白结合率的测定  

Determination of plasma protein binding rate of diphenylpi-(2, 4-dichlorbenzohydroxamato) tin in rats

作　　者：郭璞[1] 李云兰[1] 李青山[1] 

机构地区：[1]山西医科大学药学院,山西太原030001

出　　处：《药物评价研究》2013年第3期176-179,共4页Drug Evaluation Research

基　　金：国家"重大新药创制"科技重大专项(2009ZX09103-104);国家自然科学基金资助项目(30973603);山西省归国人员基金(2010-54);山西医科大学08博士启动基金

摘　　要：目的建立二-(2,4-二氯苯甲酰异羟肟酸)二苯基合锡(DPDCT)在大鼠血浆中蛋白结合率的测定方法。方法采用HPLC法测定大鼠血浆中药物总浓度及游离药物浓度,结合平衡透析法测定血浆蛋白结合率。结果 DPDCT在质量浓度为0.2、1.0、5.0μg/mL时与大鼠的血浆蛋白结合率分别为(58.9±2.0)%、(57.4±1.2)%和(58.6±0.8)%。不同浓度组血浆蛋白结合率差异无统计学意义。结论该方法灵敏度高,重复性好,操作简单,能够满足生物样品分析的要求。DPDCT具有中等强度的血浆蛋白结合率。Objective To develop a method for the determination of the protein binding rate of diphenylpi-（2, 4-dichlorbenzohydroxamato） tin （IV） （DPDCT） in rat plasma. Methods The HPLC combined with equilibrium dialysis was carried out to determine the plasma protein binding rate of DPDCT. Results The plasma protein binding rates in rats were（58.9 ± 2.0）%, （57.4 ± 1.2）%, and （58.6 ± 0.8）% when the concentration of DPDCT was 0.2, 1.0, and 5.0 μg/mL, respectively. There was no significant difference in all groups. Conclusion The method is simple, rapid, accurate, and sensitive, and it could meet the requirement for analysis method of biological samples. DPDCT has medium plasma protein binding rates.

关 键 词：二-(2 4-二氯苯甲酰异羟肟酸)二苯基合锡 HPLC 平衡透析法 血浆蛋白结合率 

分 类 号：R969.1[医药卫生—药理学]