脱氧核酶在细菌研究领域的现状与展望  

Status and prospect of deoxyribozyme in the research field of bacterial

作　　者：王同慧[1] 凌保东[2] 

机构地区：[1]川北医学院药物研究所暨药理学教研室,南充637007 [2]成都医学院药物研究所暨药理学教研室,成都610500

出　　处：《中国抗生素杂志》2013年第7期487-491,共5页Chinese Journal of Antibiotics

基　　金：四川省重点科技资助项目(2013jy0065)

摘　　要：通过体外分子进化技术合成的具有催化活性和识别能力的单链DNA分子,称为脱氧核酶。其中10-23型脱氧核酶是应用最为广泛的脱氧核酶之一,除了在抗肿瘤、抗病毒、遗传性疾病等方面应用广泛外,近年来在细菌耐药研究领域也越来越受到关注。本文主要从10-23型脱氧核酶的结构及作用靶位的特点、切割反应的原理、设计标准与化学修饰方法及其在细菌研究领域的研究现状等几个方面进行介绍。The single stranded DNA molecule with catalytical activity and the recognition ability, which is synthesized by molecular evolution technique in vitro, is called deoxyribozyme. 10-23 deoxyribozyme is one of the most widely used deoxyribozymes. It＇s not only widely used in antitumor, antiviral, genetic disease and so on, but also attracted more and more attention in the research field of bacterial resistance in recent years. This paper will introduce 10-23 deoxyribozyme mainly from several aspects, such as the structure and target characteristics, cutting principle, design standard, chemical modification method and the research status in the research field of bacteria.

关 键 词：反义技术 10-23型脱氧核酶 基因沉默 

分 类 号：R378[医药卫生—病原生物学]