微波诱变及添加氯化镧筛选阿扎霉素B高产菌株  

Breeding of high azalomycin B–producing strain by microwave radiation and lanthanum chloride adding

作　　者：穆云龙[1] 丁彦博[1] 蔡超靖[1] 单越琦[1] 可爱兵[1] 霍培元[1] 朱京童[1] 路新华[1] 

机构地区：[1]微生物药物国家工程研究中心,河北省工业微生物代谢工程技术研究中心,华北制药集团新药研究开发有限责任公司,石家庄050015

出　　处：《中国抗生素杂志》2013年第7期498-500,535,共4页Chinese Journal of Antibiotics

基　　金：国家科技重大新药创制专项(No.2010ZX09401-403);石家庄国际科技合作计划项目(编号09120016A)

摘　　要：目的拟通过微波诱变和添加氯化镧对阿扎霉素B产生菌N98．1634进行选育，获得高产菌株。方法对菌株进行40-240s的微波诱变，采用金黄色葡萄球菌抑菌圈法获得初筛通过株，并进行HPLC复筛获得高产菌株；在高产菌株发酵培养基中加入5-30mg／L的氯化镧考查其对阿扎霉素B合成的影响。结果通过微波诱变选出1株阿扎霉素B产量达到945．0μg／mL的突变菌株MW-638，较出发菌株N98-1634的752．4μg／mL提高了25．6％，随后经过添加15mg／L氯化镧阿扎霉素B单位最终达到1413．8μg／mL。结论微波诱变对提高菌株N98-1634阿扎霉素B发酵单位效果明显，诱变菌株添加适量氯化镧可以显著提高其产量。Objective To screen high azalomycin B-producing strains by microwave radiation and adding lanthanum chloride. Methods After microwave radiation mutated the strains with 40 to 240 seconds, preliminary positive strains were picked out by measurement of the inhibitory activity to the Staphylococcus aureus. And then subsequent determination by HPLC were carried out for the high azalomycin B-producting strains. Then lanthanum chloride was added in fermentation medium of the strain with the concentration of 5-30mg/L. Results The strain MW-638 was got by microwave radiation producing azalomycin B with the yield of 945.0μg/mL which raised by 25.6% than the control. The production of MW-638 reached up to 1413.8gg/mL eventually by adding the concentration of 15mg/L lanthanum chloride. Conclusion Microwave radiation and lanthanum chloride adding could significantly improve the yield of azalomycin B.

关 键 词：微波 氯化镧 阿扎霉素B 选育 

分 类 号：R978.1[医药卫生—药品]