金属离子对红霉素合成及组分转化的影响及机理分析  

Effects and mechanism analysis of metal ions on biosynthesis and component transformation of erythromycin

作　　者：赵腾[1] 高淑红[1] 陈长华[1] 叶蕊芳[1] 张增辉[2] 李强[2] 孙玉洁[2] 

机构地区：[1]生物反应器工程国家重点实验室,华东理工大学生物工程学院,上海200237 [2]河南天方药业股份有限公司,驻马店463000

出　　处：《中国抗生素杂志》2013年第7期507-511,共5页Chinese Journal of Antibiotics

摘　　要：目的研究Co2+、Zn2+和Na2MoO43种金属离子对糖多孢红霉菌发酵生产红霉素及组分转化的影响,并对其影响机理进行相关分析。方法通过摇瓶实验确定金属离子最优配比,并在15L发酵罐上放大;通过菌体在合成培养基中的摇瓶发酵研究红霉素合成途径中羟基化基因eryK、甲基化基因eryG的表达量与红霉素合成及组分转化的关系。结果 3种金属离子的添加使红霉素发酵单位由对照罐8433U/mL提高到11858U/mL,有效组分ErA由对照83.6%提高到89%;eryK和eryG两种基因的表达量显著提高,且eryK/eryG比值与有效组分ErA含量具有较好的相关性。结论 3种金属离子能通过调节菌种相关基因的表达,对红霉素的合成及组分转化产生促进作用。Objective To study effects and mechanism analysis of Co^2＋、Zn^2＋ and Na2MoO4 on biosynthesis and component transformation of erythromycin by Streptomyces erythraea. Methods The optimal ratio of metal ions was obtained through shake flask experiments, and the culture process was performed in 15L fermentation tank successfully. At the same time, the expression of hydroxylation gene eryK and methylation gene eryG were also studied in shake flask ofStreptomyces erythraea. Results The final titer was improved from 8433U/mL（control） to 11858U/mL, and the relative percentage of erythromycin A increased from 83.6% to 89% in 15L fermentation tank using the optimal combination of metal ions in shake flask. The addition of metal ions can improve the gene expression of eryK and eryG. Further more, the radio of eryK/eryG had a relatively close relationship with the components of erythromycin. Conclusion It was suggested that stimulating the transcription of related genes by metal ions may play an important role in the biosynthesis and component transformation of erythromycin.

关 键 词：糖多孢红霉菌 红霉素 金属离子 组分转化 基因表达 

分 类 号：Q936[生物学—微生物学]