大白猪TLR4基因外显子1遗传变异及其与细胞因子水平的关系  

The Genetic Variation of TLR4 Gene Exon 1 and Its Correlation with Cytokines Level in Large White Pigs

作　　者：苏先敏[1] 王靖[1] 赵乔辉[1] 刘璐[1] 吴圣龙[1,2] 包文斌[1,2] 

机构地区：[1]扬州大学动物科学与技术学院,江苏省动物遗传繁育与分子设计重点实验室,江苏扬州225009 [2]江苏省现代种猪分子选育工程技术研究中心,江苏常州213149

出　　处：《中国畜牧杂志》2013年第13期1-4,共4页Chinese Journal of Animal Science

基　　金：江苏省研究生科研创新计划(CXLX12-0927);江苏省产学研联合创新基金(BY2012157);苏州市科技支撑(SN201013;SN201133);常州市科技攻关项目(CE20102006)

摘　　要：本实验运用PCR-SSCP技术对大白猪TLR4基因外显子1遗传变异进行分析,同时利用ELISA方法对外周血重要细胞因子(IL-2、IL-4、IL-6、IFN-Beta、IL-10、IL-12)的水平进行测定,旨在分析大白猪TLR4基因外显子1多态性及其与重要细胞因子水平间的关系,探讨将其作为抗F18大肠杆菌病遗传标记的可行性。结果表明:大白猪TLR4基因外显子1存在G93C同义突变位点,共检测到3种基因型,分别定义为BB、BC和CC;关联分析结果表明,大白猪TLR4外显子1变异位点对机体重要细胞因子水平没有显著影响(P>0.05),表明基于该位点的分子选育不会对机体免疫应答和一般抗病力产生不利影响。结果提示,有必要将TLR4基因外显子1的变异位点作为抗大肠杆菌病的重要遗传标记进行深入研究。This study was conducted to analyze genetic variation of exon 1 of TLR4 gene by PCR-SSCP and determine level of the vital cytokines （IL-2, L-4, IL-6, IFN-Beta, IL-10, IL-12） using ELISA in Large White pigs, aimed to analyze polymorphism of exon 1 of TLR4 gene and its association with significant cytokines level, discussing feasibility of the polymorphism loci as genetic markers with resistance to E. coli F18. The results showed that there were BB, BC and CC genotypes in Large White pigs, while a G93C synonymous mutation was found in BB genotype. The correlation analysis indicated that genetic variation of exon 1 of TLR4 gene had no significant affected on the important cytokines level （P〉0.05）, and molecular breeding based on the point could not make unfavorable influence on immune response and resistance to disease, which indicated that the polymorphism loci as crucial genetic markers with resistance to E. coli F18 was worthy of further study.

关 键 词：TLR4基因 多态性 细胞因子 大白猪 

分 类 号：S828.2[农业科学—畜牧学]