不同锌源和锌水平对猪IPEC-J2细胞GLP-2基因表达的影响  

Effects of Different Zinc Sources and Levels on GLP-2 Expressions in IPEC-J2 Cells

作　　者：计乔平[1] 余冰[1] 韩国全[1] 虞洁[1] 郑萍[1] 何军[1] 毛湘冰[1] 黄志清[1] 陈代文[1] 郭荣富[2] 

机构地区：[1]四川农业大学动物营养研究所,动物抗病营养农业部重点实验室,四川成都611130 [2]云南农业大学,云南省动物营养与饲料重点实验室,云南昆明650201

出　　处：《中国畜牧杂志》2013年第13期35-38,共4页Chinese Journal of Animal Science

基　　金：现代农业产业技术体系建设专项资金资助(CARS-36);教育部长江学者和创新团队发展计划项目资助(IRTO555)

摘　　要：本试验旨在探讨不同锌源及锌水平对猪小肠上皮细胞(IPEC-J2)胰高血糖素样肽2(GLP-2)表达的影响。分别以乳酸锌、硫酸锌(锌浓度分别为50、100、150、200 mg/L)作用IPEC-J2细胞,实时荧光定量RT-PCR方法检测GLP-2 mRNA表达,以β-actin mRNA水平作为内参对照。结果表明:在6、12 h检测时间点,不同锌源和水平及其互作对GLP-2 mRNA表达影响极显著(P<0.01);在24 h时不同锌源对其表达影响极显著(P<0.01),锌添加水平对其表达影响显著(P<0.05),不同锌源与锌添加水平交互作用则不显著(P<0.05);乳酸锌、硫酸锌促进GLP-2基因mRNA表达均具有正向浓度效应。乳酸锌和硫酸锌均可上调肠道细胞GLP-2基因mRNA的表达;在同等锌浓度水平下,乳酸锌促进GLP-2基因表达的效果优于硫酸锌。This experiment approached the effects of zinc sources and levels on GLP-2 expression in swine intestinal epithelium. IPEC-J2 cells were cultured and treated with different concentrations （100,50, 150,200 rag/L） of zinc lactate and zinc sulfate. The gene expression of GLP-2 was determined with Real-time RT-PCR using β-action as internal control. The effects of zinc sources, levels and their interaction on GLP-2 expressions in IPEC-J2 cells were significant （P〈0.01） as well. Zinc sources significantly improved the expressions of GLP-2 mRNA after 24 h （P〈0.01）. Zinc concentrations significantly increased GLP-2 expressions in IPEC-J2 cell （P〉0.05）, however, the interaction between zinc sources and levels was not significant （P〉0.05）. It was demonstrated that zinc lactate and zinc sulfate both increased the gene expressions of GLP-2 in IPEC-J2 cells, and the effect of zinc lactate on GLP-2 expression was better than zinc sulfate at same concentration.

关 键 词：乳酸锌 硫酸锌 IPEC-J2 GLP-2 猪 

分 类 号：S828.5[农业科学—畜牧学]