NODs/RIP2信号通路关键基因在断奶仔猪不同组织中的mRNA表达  

The mRNA Expression of the Key Genes in NODs/RIP2 Signaling Pathway in Different Tissues of Weaned Piglets

作　　者：陈逢[1] 刘玉兰[1] 李权[1] 朱惠玲[1] 侯永清[1] 魏贤斌 易丹 陈洪波[1] 

机构地区：[1]武汉轻工大学,动物营养与饲料科学湖北省重点实验室,湖北武汉430023 [2]湖北仙桃市动物卫生监督所,湖北仙桃433099

出　　处：《中国畜牧杂志》2013年第13期39-42,共4页Chinese Journal of Animal Science

基　　金：教育部新世纪优秀人才支持计划(NCET-10-0158);国家自然科学基金面上项目(31172222);湖北省自然科学基金重点项目(2010CDA050);湖北省国际科技合作项目(2011BFA008);湖北省教育厅科学技术研究计划优秀中青年人才项目(Q20111702);武汉市科技计划项目(201171034320)

摘　　要：本试验旨在研究核苷酸结合寡聚化结构域蛋白/受体相互作用蛋白2(NODs/RIP2)信号通路关键基因在断奶仔猪不同组织的分布情况。选择12头杜×长×大断奶仔猪,屠宰,取脾脏、胸腺、肠道淋巴结、下丘脑、垂体、肾上腺、肝脏、腓肠肌、皮下脂肪、空肠和回肠组织。应用实时荧光定量PCR技术测定NODs/RIP2信号通路关键基因,包括NOD1、NOD2和RIP2在各组织的mRNA表达水平。结果表明:NOD1、NOD2和RIP2在所检测的11个组织中均有表达。NOD1 mRNA在肠道淋巴结和下丘脑表达量较高;NOD2 mRNA在肠道淋巴结、脾脏、下丘脑和胸腺表达量较高;RIP2 mRNA在肠道淋巴结、胸腺和脾脏表达量较高。NODs/RIP2信号通路关键基因在不同组织中表达差异较大,可能与仔猪各组织对病原的识别和抵抗能力有关。This experiment was conducted to investigate the mRNA expression pattern of the key genes in nucleotide- binding oligomerization domain proteins/ receptor-interacting protein 2 （NODs/RIP2） signaling pathway in different tissues of weaned piglets. Twelve crossbred （DurocxLarge WhitexLandrace） piglets were sacrificed for collection of spleen, thymus, mesenteric lymph node, hypothalamus, pituitary gland, adrenal gland, liver, gastrocnemius, subcutaneous fat, jejunum and ileum. The mRNA expression levels of the key genes in NODs/RIP2 signaling pathway, including NOD1, NOD2 and RIP2 in different tissues were detected by real-time quantitative PCR. The result showed that the mRNA of these key genes was expressed in all of 11 tissues. NOD1 mRNA expression level was high in mesenteric lymph node and hypothalamus; NOD2 mRNA expression level was high in mesenteric lymph node, spleen, hypothalamus and thymus; RIP2 mRNA expression level was high in mesenteric lymph node, thymus and spleen. These results indicate that there are significant differences on the mRNA expression of the key genes in NODs/RIP2 signaling pathway in different tissues, which may be associated with the ability of pigs to recognize pathogens.

关 键 词：NODs RIP2信号通路 实时荧光定量PCR 断奶仔猪 

分 类 号：S828.5[农业科学—畜牧学]