5个黑山羊品种(群体)微卫星标记的遗传多样性研究  

Genetic Diversity among Five Black Goat Breeds(Populations) Based on Microsatellite Analysis

作　　者：武志娟[1] 陈明华[2] 万洁[2] 韦雷飞 钟金城[1] 李娜[1] 姜雪鸥[1] 

机构地区：[1]西南民族大学,动物遗传育种学国家民委-教育部重点实验室,四川成都610041 [2]攀枝花市农林科学研究院,四川攀枝花617061

出　　处：《中国畜牧兽医》2013年第6期146-152,共7页China Animal Husbandry & Veterinary Medicine

基　　金：攀枝花市重点产业科技支撑项目(2010CY-N-1)

摘　　要：利用14个微卫星标记分析了攀枝花地方黑山羊、建昌黑山羊、云岭黑山羊、金堂黑山羊、乐至黑山羊5个品种(群体)的遗传多样性及亲缘关系。结果显示,14个微卫星座位多态信息含量(PIC)在0.5129～0.7646之间,均为高度多态位点;5个黑山羊品种(群体)的平均多态信息含量(PIC)为0.6405～0.6856,平均杂合度(H)在0.6985～0.7371之间,说明遗传多样性丰富;群体间平均遗传分化系数(Fst)为0.0296,说明群体间的变异仅为2.96%,群体间存在基因交流;攀枝花黑山羊和金堂黑山羊2个群体之间的Nei氏遗传距离最大(D=0.1286),金堂黑山羊和乐至黑山羊之间的遗传距离最小(D=0.0365);NJ聚类图中,攀枝花黑山羊、建昌黑山羊和云岭黑山羊聚为一类,金堂黑山羊和乐至黑山羊聚为一类,聚类结果与群体的地理分布较为一致。Genetic diversity for Pangzhihua Black goat （PZH） and other four black goat breeds（populations） ,including Jan- chang Black goat （JC） ,Yun[ing Black goat （YL） ,Jintar^g Black goat （JT）, Lezhi Black goat （LZ）. We reestimated using four- teen microsatellite makers by PCR, polyacrylamide gel electrophoresis, and silver staining of modified Sanguintti method. The results showed that all of the microsatellites were highly polymorphic loci, the polymorphic information content （PIC） varied from 0. 5129 to 0. 7646. Each of the five breeds（populations） possesses had a high level of genetic variability, the PIC and the mean heterozygosity （H） varied from 0. 6405 to 0. 6856 and 0. 6985 to 0. 7371 ,respectively. But the Wright^s F-statistics of subpopulation within total （Fst） showed a low mean value 0. 0296, suggesting the variation between populations was only 2.96 ~ share and gene flow was exist among groups. Nei^s genetic distances （D） were calculated, which varied from 0. 0365 （between JT and LZ） to 0. 1286（between PZH and JT）. In the NJ cluster analysis, PZH, JC and YL could be separated to one cluster, JT and LZ to the other. Additionally, the result displayed a remarkable degree of consistency with their different geo- graphical origins.

关 键 词：黑山羊 微卫星 遗传多样性 

分 类 号：S827.2[农业科学—畜牧学]