奶牛瘤胃液中浸麻类芽孢杆菌的分离鉴定  

Isolation and Identification of Paenibacillus macerans Strain from Cows Rumen Fluid

作　　者：邓慧媛[1,2] 李志忠[2] 张凯[1] 李建喜[1] 

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所,甘肃兰州730050 [2]兰州理工大学生命与工程学院,甘肃兰州730050

出　　处：《中国畜牧兽医》2013年第6期246-249,共4页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(31072162);现代农业奶牛产业技术体系(CARS-37-06)

摘　　要：采用平板划线分离法从健康奶牛瘤胃液中分离得到1株细菌Y-1,体外模拟瘤胃环境增殖Y-1,进行形态学观察、生化反应和16SrDNA基因同源性比较,并对其进行系统发育树的构建等系统鉴定。结果表明,该细菌为浸麻类芽孢杆菌(Paenibacillus macerans),本研究还对瘤胃液中存在该菌的原因做了分析。We used flat crossed separation method to get a bacterial Y-l, which was isolated from a cow rumen fluid. Simu- lating rumen environment in vitro to proliferate Y-1. The test adopted the methods, which include morphological observation, biochemical reactions and 16S rDNA gene homology comparison, and constructed the phylogenetic tree for Y-1. The results in- dicated that Y-1 was Paenibacillus macerans strain, and analyzed the reasons for the presence of bacteria in the rumen fluid.

关 键 词：浸麻类芽孢杆菌 形态学鉴定 生化管鉴定 16S rDNA测序 

分 类 号：S823[农业科学—畜牧学]