基因芯片技术筛选哮喘大鼠差异表达基因的研究  

Differential expression of asthma related genes in rat scaned by gene chip

作　　者：刘鑫[1] 熊花[2] 刘斌[2] 王小莉[2] 罗晓青[2] 黄艳[1] 

机构地区：[1]南华大学附属第一医院,湖南衡阳421001 [2]南华大学,湖南衡阳421001

出　　处：《中国现代医学杂志》2013年第10期10-15,共6页China Journal of Modern Medicine

基　　金：湖南省中医药管理局资助课题(No:201249)

摘　　要：目的利用基因芯片技术寻找哮喘大鼠与正常大鼠之间差异基因的动态变化,寻找参与哮喘发病的新基因。方法分别于激发哮喘2、4、8周处死大鼠收集肺泡灌洗液行炎性细胞及嗜酸性粒细胞计数,取肺组织行HE染色、病理图像分析,提取肺组织RNA行基因芯片筛选及实时定量PCR验证。以基因表达倍数值2.0和基因表达倍数值-2.0为阈值来确定差异表达基因,然后用GO及Pathway分析对差异表达基因进行功能分类分析,结果以P<0.05为差异显著性标准。结果从24 358条表达基因谱中,筛选出哮喘大鼠与正常大鼠持续性差异表达2倍以上的基因有13条,其中发现Mal和TPD52L1等新基因参与哮喘疾病过程。结论 Mal、TPD52L1基因在肺组织中异常表达,影响哮喘的病程进展。[ Objective ] To study the dynamic difference of gene expressions between asthma rats and normal rats and screen novel asthma-associated genes by gene chip. [Methods ] The rats with asthma induced were executed in 2, 4 and 8 weeks, respectively, HE staining and pathology image analysis was performed on lung tissue, brou- choalveolar lavage fluid （BALF） was gathered to count the number of inflammation cells and eosinophil cells, lung tissue RNA was extracted and gene-chip technology was used to screen the novel asthma-associated genes, which was then confirmed by the real time RT-PCR. Gene expression times value 2.0 and -2.0 for threshold was used to determine the differential expression genes, then GO and pathway analysis were used to analyze the function and classification of differential expression genes. Results are according to the P 〈0.05 significance of difference stan- dard. [ Results ] The results showed that 13 persistently expressed genes were screened from 24358 gene expression profiles. And new genes Mal,TPD52L1 and other genes were involved in the procession of asthma. [ Conclusion] The abnormal expression of Mal and TPD52L1 genes in lung tissue affect the procession of asthma.

关 键 词：哮喘 基因芯片 MAL TPD52L1 

分 类 号：R562.25[医药卫生—呼吸系统] R-332[医药卫生—内科学]