响应面法优化核桃花粉黄酮提取工艺  

Optimization of Extraction of Total Flavonoids from Walnut Staminate Flowers by Response Surface Design

作　　者：王丹[1] 翟梅枝[1] 史冠昭[1] 毛光瑞[1] 

机构地区：[1]西北农林科技大学林学院,陕西杨凌712100

出　　处：《安徽农业科学》2013年第12期5470-5474,共5页Journal of Anhui Agricultural Sciences

摘　　要：[目的]优化核桃花粉黄酮提取工艺。[方法]试验先采用溶剂提取法提取核桃花粉中的黄酮物质,并在单因素试验的基础上,以总黄酮得率为响应值,采用响应面试验设计方法优化核桃花粉总黄酮提取工艺。[结果]响应面法得出的提取核桃花粉黄酮的最佳工艺参数如下:乙醇浓度64.7%,提取温度80℃,液料比为30∶1 ml/g,提取时间为102 min,该工艺条件可使总黄酮得率达4.17%。[结论]响应面法对黄酮提取的优化具有实际应用价值,可为工业化利用丰富的核桃花粉资源提取总黄酮提供生产依据。[Objective] To optimize extraction technique for flavonoids from walnut staminate flowers.[Method]Total flavonoids were extracted from walnut staminate flowers with solvent method.Based on single-factor test,response surface analysis was conducted to optimize the extraction technology of total flavonoids.The procedure for the extraction was performed with the recovery yield of flavonoids as the response.[Result]The optimum extraction conditions were confirmed by response surface method as follows： ethanol concentration 64.7% temperature 80 ℃ liquid to material ratio 30 ∶ 1 and time 102 min.The recovery yield of flavonoids got to 4.17% once extraction.[Conclusion] RSD method has practical application value for optimization of flavonoids extraction,which will provide production basis for extracting total flavonoids from walnut staminate flower.

关 键 词：响应面 核桃花粉 黄酮 

分 类 号：S664.1[农业科学—果树学]