青海地区子痫前期患者血清可溶性人类白细胞相关抗原G检测及预诊指标的建立  

THE DETECTION OF SHLA-G IN PRE-ECLAMPSIA TO ESTABLISH PRELIMINARY EXAMINATION INDEX AT QINGHAI PLATEAU

作　　者：谢莹莺[1] 李凤莲[1] 赵生珠[1] 杨娟[1] 

机构地区：[1]青海大学附属医院,青海西宁810001

出　　处：《青海医学院学报》2013年第2期133-136,共4页Journal of Qinghai Medical College

摘　　要：目的总结青海地区子痫前期患者血清可溶性人类白细胞相关抗原G的特征,寻找子痫前期的预诊指标。方法收集34例重度子痫前期患者、26例轻度子痫前期患者和30例正常足月妊娠妇女的产前血清样品,应用双抗体夹心酶联免疫吸附法检测血清中可溶性HLA-G的表达水平并行相关分析。结果 (1)重度子痫前期与轻度子痫前期患者血清中sHLA-G浓度均低于正常足月妊娠组,差异有统计学意义(P<0.05),轻重度之间差异亦有统计学意义(P<0.05)。(2)利用sHLA-G的ROC曲线界定值为848.665 ng/L,可以将该值作为诊断子痫前期的初筛指标。结论子痫前期血清sHLA-G浓度可能与子痫前期发病及病情轻重程度相关,血清sHLA-G拟可作为子痫前期初筛预诊指标。Objective To obtain a diagnostic biomarker and some information concerning intervention of the pre- eelampsia, the soluble human leukocyte antigen -G （sHLA -G）was compared between normal full -term pregnancy and pre - eclampsia in Qinghai plateau. Methods The serum sHLA - G, in 34 patients with the severe pre- eclampsia,26 patients with the mild pre -eclampsia,30 normal pregnant women, respectively, were detected by a validated sandwich enzyme -linked immunosorbent assay （ELISA）. Result ①Serum sHLA - G values of the severe pre - eclampsia group and the mild group were obviously higher than that of the normal control group （ P 〈 0.05）. There was significant difference between the severe group and the mild group （P 〈 0.05 ）. ②Cut off value was 848. 665 ng/L defined by receiver operating characteristic curve （ ROC curve）. Conclusion The serum sHLA -G obviously decreases in pre -eclampsia, which is probably related to this disease development. The sHLA - G may be regarded as a good biomarker for the diagnostic screening.

关 键 词：青海地区 子痫前期 可溶性人白细胞抗原G 预诊指标 

分 类 号：R714.245[医药卫生—妇产科学]