脂质微泡与PLGA纳米粒复合体的制备原理分析  

Analysis of the preparation principle of the complex of lipid ultrasound microbubbles and PLGA nanoparticles

作　　者：张亚萍[1] 冉海涛[2] 

机构地区：[1]内江市第一人民医院特检科,四川省641000 [2]重庆医科大学附属第二医院超声影像学研究所

出　　处：《中华临床医师杂志（电子版）》2013年第9期133-135,共3页Chinese Journal of Clinicians(Electronic Edition)

基　　金：国家自然科学基金面上项目(30770565)

摘　　要：目的制备一种连接有PLGA纳米粒的新型脂质超声微泡并分析其制备原理。方法采用碳二亚胺法,并结合静电吸附,使表面带有氨基和正(或负)电荷的脂质微泡与表面带(或不带)有活化羧基的PLGA纳米粒进行缩合偶联反应,通过普通光学显微镜和荧光显微镜观察两者结合情况。结果普通光镜与荧光显微镜下观察,表面带正电荷的脂质微泡与活化羧基的PLGA纳米粒可以成功偶联,PLGA纳米粒结合于脂质微泡表面;而表面带负电荷的脂质微泡与活化羧基的PLGA纳米粒以及表面带正电荷的脂质微泡与未活化羧基的高分子聚合物纳米粒均未见结合。结论化学共价偶联与静电吸附协同作用有利于促成脂质微泡与PLGA纳米粒的有效连接,而两者单用均不能产生连接。Objective To analyze the preparation principle of a novel kind of lipid ultrasound microbubble connected PLGA nanopartieles on the surface. Methods Through the methods of carbodiimide and electrostatic adsorption,lipid microbubbles of positive charge （ or negative charge ） with aminogen On the surface and PLGA nanoparticles with （ or without ） activated earboxyl on the surface were mixed to produce coupling reaction. The situation of combination was observed through light microscope and fluorescence microscope, respectively. Results Under the light microscope and fluorescence microscope, lipid microbubbles of positive charge with aminogen on the surface and PLGA nanopartieles with activated carboxyl on the surface could occur coupling reaction successfully, and it could be seen that PLGA nanoparticles were connected on the surface of lipid microbubbles ; while the other two methods couldn＇t produce coupling reaction. Conclusion Combined with synergy of chemical covalent coupling and electrostatic adsorption can facilitate lipid microbubbles effectively connected with PLGA nanoparticles, while neither of them can success in connection separately.

关 键 词：纳米球 膜脂质类 碳二亚胺法 偶联反应 连接 

分 类 号：R944[医药卫生—药剂学]