斯氏假单胞菌对草鱼养殖水体水质的调控作用  

Effect of Pseudomonas stutzeri F1M on water quality in grass carp culture

作　　者：郑佳佳[1] 张小平[1] 胡彩虹[1] 李卫芬[1] 

机构地区：[1]浙江大学动物科学学院饲料研究所,浙江杭州310058

出　　处：《渔业现代化》2013年第3期5-9,共5页Fishery Modernization

基　　金：公益性行业(农业)科研专项:淡水池塘工程化改造与环境修复技术研究与示范-池塘养殖污染评价及利用微生物调控养殖水质的技术(201203083);国家重点基础研究发展计划(973计划)项目:有益微生物对养殖水环境的调控作用及机理(2009CB118705)

摘　　要：为研究斯氏假单胞菌(Pseudomonas stutzeri F1M)对草鱼养殖水质的调控作用,进行了室外试验。试验选择初始体重为(5.0±0.5)g的草鱼2 400尾,随机分为4组,每组3个重复,每7 d分别向水中添加0、2×107、1×108和2×108cfu/L斯氏假单胞菌,各组投喂相同饲料。试验期为15 d,每3 d测定亚硝酸盐氮、硝酸盐氮、氨氮、总氮、正磷酸盐、总磷等水质指标。结果显示,斯氏假单胞菌用量1×108cfu/L和2×108cfu/L均可有效降解水体亚硝酸盐氮和总无机氮,第15天时,亚硝酸盐氮和总无机氮浓度分别比对照组减16.8%、15.3%和49.5%(P<0.01)、51.8%(P<0.01)。表明斯氏假单胞菌调控草鱼养殖水体水质的最适添加量为1×108 cfu/L。This experiment was conducted to study the application of Pseudomonas stutzeri F1M for water quality control in grass carp culture. A total of 2 400 grass carps weighing （5.0 ± 0. 5 ） g were randomly allotted to 4 groups. Each group consisted of 3 replications. Pseudomonas stutzeri F1 M were added to the water with 0,2 ×10^7 ,1 ×10^8 or 2 ×10^8 cfu/L every 7 days, respectively. Grass carps in all groups were fed the same feed. The trial period was 15 days. Nitrite nitrogen, nitrate nitrogen, ammonia, total inorganic nitrogen, total nitrogen, orthophosphate, total phosphorus and other water quality indicators were measured every 3 days. The results showed that 1 ×10^8 and 2 ×10^8 cfu/L Pseudomonas stutzeri F1M could effectively degraded nitrite nitrogen and total inorganic nitrogen by 16.8%, 15.3% and 49.5% （ P 〈 0.01 ）, 51.8% （P 〈0. 01 ）at the 15th day, respectively, compared with control. It is suggested that the optimum dosage of Pseudomonas stutzeri FI M regulating aquatic water was 1 ×10^8 cfu/L.

关 键 词：斯氏假单胞菌(Pseudomonas stutzeriF1M) 草鱼 水质 水产养殖 

分 类 号：S949[农业科学—水产养殖] S965.112[农业科学—水产科学]