绿原酸对体外培养成骨细胞活性的影响  

Effects of Chlorogenic Acid on the Activity of Cultured Osteoblasts in vitro

作　　者：王朝元[1] 易继凌[1] 宋超[1] 魏甜甜[1] 唐俊龙[1] 杨光忠[2] 

机构地区：[1]中南民族大学生命科学学院,武汉430074 [2]中南民族大学药学院,武汉430074

出　　处：《中南民族大学学报（自然科学版）》2013年第2期46-50,共5页Journal of South-Central University for Nationalities：Natural Science Edition

基　　金：湖北省自然科学基金资助项目(2009CDZ033)

摘　　要：为研究接骨草成分之一绿原酸(CGA)对成骨细胞MC3T3-E1活性的影响,采用MTT法检测11.02,22.05,44.09,88.19μmol/L CGA对成骨细胞增殖率,碱性磷酸酶(ALP)试剂盒检测成骨细胞ALP活性,实时定量PCR检测成骨细胞骨相关基因的表达.结果表明:11.02～88.19μmol/L CGA对成骨细胞增殖具有明显的促进作用.培养2d,44.09μmol/L CGA能促进ALP表达上调;22.05,44.09μmol/L CGA能促进ALP、Runx2、Osterix、c-jun和c-fos基因的表达;培养6 d,44.09μmol/L CGA能促进c-fos基因的表达;在整个培养过程中,I型胶原(Col-I)基因的表达具有浓度和时间依赖性.故CGA具有一定的成骨活性,是接骨草的成骨活性成分之一.To investigate the effect of chlorogenic acid （ CGA）, one of the components of Sambucus chinensis, on the osteogenic activity of cultured osteoblasts, osteoblasts were treated with 11.02,22.05, 44.09, 88. 19 p^mol/L CGA, respectively. MTr assay, alkaline phosphate（ALP） kit, real-time PCR were used to measure the proliferation rate, ALP activity and expression of bone-related genes in osteoblasts. The results showed that 11.02 - 88.19 jxmol/L CGA could promote the proliferation rate remarkably. The activity of ALP in osteoblasts was up-regulated when treated with 44.09μmol/L CGA for 2 d. The expression of bone-related genes such as ALP,Runx2 ,Osterix,c-jun and c-fos could be enhanced when treated with 22.05,44.09 μmol/L CGA for 2 d. The expression of c-los could be promoted when treated with 44.09 p, mol/L CGA for 6 days. In the whole process, the expression of Col-I was increased in a time-dependent and concentration-dependent manner. Therefore, CGA showed osteogenic activity , which demonstrated that it was one of the osteogenic active ingredients of Sambucus chinensis.

关 键 词：绿原酸 成骨细胞 碱性磷酸酶活性 成骨分化相关基因 

分 类 号：Q813.1[生物学—生物工程] Q786