胶州湾海域PAHs污染与可培养半知菌的多样性  

Culturable deuteromycetes diversity and PAHs contamination in Jiaozhou bay

作　　者：姜会超[1,2] 田黎[1,2] 史振平[1] 张久明[1] 

机构地区：[1]青岛科技大学,山东青岛266042 [2]国家海洋局第一海洋研究所,山东青岛266061

出　　处：《海洋科学》2013年第5期33-38,共6页Marine Sciences

基　　金："十二五"国家科技支撑计划项目(2011BAE06B04);国家863计划项目(2011AA10A202-2;2011AA09070402);海洋公益项目(201005032-2)

摘　　要：用5种不同的培养基对胶州湾近岸污染环境可培养半知菌进行选择性分离,共得到235株半知菌,其中YM培养基、MPDA培养基对半知菌的分离效果最好,分别占分离总量的26.4%和24.7%。试验选择的3个站位:石老人潮间带、海泊河入海口、李村河入海口PAHs含量存在显著性差异(P<0.05),其中石老人最低,平均为113.8 ng/g,海泊河PAHs为324.9 ng/g,介于中等水平,李村河含量最高为882.1 ng/g。可培养半知菌群体随PAHs含量的增加表现出先升高后降低的趋势。对各站位的半知菌进行统计发现,污染不同的站位,可培养半知菌优势种群不同,说明PAHs的污染可对半知菌的菌落结构组成产生影响。Five different kinds of culture medium were used to selectively isolate culturable Deuteromycetes in contaminated coast of Jiaozhou bay and 235 strains of Deuteromycetes were obtained. Among the medium, YM and MPDA isolated Deuteromycetes the best. The isolate rates of the total isolated-strains of YM and MPDA were 26.4% and 24.7%, respectively. PAHs contents among the three sampling sites manifested significant difference （P〈0.05） with the lowest in Shilaoren tidal zone （average 113.8ng/g）, moderate in Haibo River estuary （average 324.9 ng/g） and the highest in Licun River estuary （average 882.1ng/g）. On the basis of the changes of PAHs con- tents in different sites, with the increase of PAHs amount, the amount of culturable Deuteromycetes increased in certain degree and then decreased. Further research showed that the dominant species differed in the three sites with different PAHs contents which indicated PAHs contamination might exert effect on the composition of Deutero- mycetes colony.

关 键 词：胶州湾 PAHS 半知菌 

分 类 号：X55[环境科学与工程—环境工程]