阿托伐他汀抑制大鼠颈动脉球囊损伤后再狭窄及对线粒体融合素2表达的影响  

Effect of atorvastatin on expression of mitofusin 2 in rats with restenosis following carotid artery balloon injury

作　　者：周炜[1] 周逸[1] 陈曼华[1] 

机构地区：[1]武汉市中心医院心内科,430014

出　　处：《中华老年心脑血管病杂志》2013年第7期762-765,共4页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：武汉市卫生局临床医学科研项目(WX12B03)

摘　　要：目的研究阿托伐他汀对大鼠颈动脉球囊损伤后再狭窄及线粒体融合素2(Mfn2)表达的影响。方法雄性Wistar大鼠48只,随机分为假手术组,对照组,阿托伐他汀1组(他汀1组)和阿托伐他汀2组(他汀2组),每组12只。他汀1组术前3d每日阿托伐他汀10mg/kg灌胃,他汀2组每日阿托伐他汀40mg/kg,随后制备颈动脉球囊损伤模型。HE染色行组织形态学观察,测量内膜面积(I)/中膜面积(M)比值;免疫组织化学及免疫印迹法检测Mfn2及增殖细胞核抗原(PCNA)表达;依文思蓝染色观察血管内皮修复。结果与假手术组比较,对照组及他汀1组和2组血管内膜增生,I/M比值显著增加(P<0.01),PCNA阳性细胞比值显著增加(P<0.01),Mfn2表达显著降低(P<0.01);与对照组比较,他汀1组和他汀2组I/M比值降低(P<0.01),PCNA阳性细胞比值降低(P<0.01),Mfn2表达升高(P<0.05);且他汀2组较他汀1组变化更显著(P<0.05)。与对照组比较,他汀1组和他汀2组依文思蓝染色区域面积与吸光度相对值的乘积显著降低(P<0.05)。结论阿托伐他汀抑制大鼠颈动脉球囊损伤后再狭窄,同时可促进内皮细胞修复,其作用可能与上调Mfn2表达有关。Objective To study the effect of atorvastatin on expression of mitoIusin 2 （Mfn2） in rats with restenosis following carotid artery balloon injury. Methods Forty-eight male Wistar rats were randomly divided into sham operation group, control group,atorvastatin groups A and B （12 in each group）. Rats in atorvasstatin groups A and B were given atorvastatin, 10mg/kg and 40 mg/kg daily,respectively,from day 3 before operation. Forteen days after a rat carotid artery bal- loon injury model was established,the rats were killed and their carotid artery tissue samples were taken. The morphology of these samples was observed with HE staining. The intima area/media area （I/M） of catrotid artery was measured. Expressions of Mfn2 and PCNA were detected by Western blott with imrnunohistochemistry staining. Repair of vascular endothelium was observed with Evans blue staining. Results The I/M,vascular endothelium proliferation rate and positive PCNA were significantly higher whereas the expression level of Mfn2 was significantly lower in control group and atorvasstatin groups A and B than in sham operation group （P〈0.01）. The I/M and positive PCNA were significantly lower whereas the expression level of Mfn2 was signif icantly higher in atorvasstatin groups A and B than in control group and in atorvasstatin group t3 than in atorvasstatin group A （P〈0.01, P〈0.05）. Conclusion Atorvastatin inhibits restenosis in rats following carotid artery balloon iniury and promotes repair of vascular enthelium by up- regulating the expression of Mfn2.

关 键 词：降血脂药 颈动脉损伤 颈动脉狭窄 线粒体 增殖细胞核抗原 内皮细胞 融合素2 

分 类 号：R96[医药卫生—药理学]