不同大孔树脂富集山茱萸中环烯醚萜苷的效果比较  

Comparison of Enrichment Effect of Iridoid Glycosides from Cornus officinalis by Different Macroporous Resins

作　　者：蔡黎明[1] 周盈[1] 蔡宝昌[1] 刘芳[1] 皮文霞[1] 

机构地区：[1]南京中医药大学,南京210046

出　　处：《中国实验方剂学杂志》2013年第13期39-41,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81274056)

摘　　要：目的:考察不同类型大孔树脂对山茱萸中环烯醚萜苷类成分的分离效果。方法:采用HPLC测定山茱萸中马钱苷及莫诺苷的含量,考察不同大孔吸附树脂分离山茱萸中环烯醚萜苷类成分的有效性。结果:马钱苷、莫诺苷的回归方程依次为Y=17.245X+13.362(r=0.999 9),Y=15.468X-41.275(r=1.000 0);线性范围分别为21.75～435,35.8～716μg;加样回收率分别为98.27%,96.29%。HPD-400型大孔树脂的分离效果最好。结论:选取马钱苷和莫诺苷含量为指标筛选富集环烯醚萜苷的大孔树脂类型是可行的,研究结果为分离山茱萸中有效部位的后续研究提供实验依据。Objective： To investigate separation effect of iridoid glycosides from Cornus officinalis by different types of macroporous resin.Method： The content of loganin and morroniside from C.officinalis was determined by HPLC,effectiveness of different macroporous adsorption resin on separating iridoid glycosides from C.officinalis was investigated.Result： HPD-400 type of macroporous resin had the best separation effect.Regression equation of loganin and morroniside were Y = 17.245X ＋ 13.362（r = 0.999 9）,Y = 15.468X 41.275（r = 1.000 0）;The linear range of them were 21.75-435,35.8-716 μg;Recoveries were 98.27%,96.29%,respectively.Conclusion： It was feasible that selected the content of loganin and morroniside as index to screen macroporous resin for enrichment iridoid glycosides from C.officinalis,this study could provide experimental basis for the follow-up study of separation of effective parts from C.officinalis.

关 键 词：山茱萸 大孔树脂 环烯醚萜苷 马钱苷 莫诺苷 

分 类 号：R283.6[医药卫生—中药学]