四妙勇安汤中哈巴苷提取率的拆方分析  

Separated Recipes Analysis on Extraction Rate of Harpagide in Simiao Yong'an Decoction

作　　者：张恩惠[1] 王翀[1] 刘斌[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《中国实验方剂学杂志》2013年第13期45-47,共3页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:考察四妙勇安汤不同拆方中哈巴苷的提取率变化,探讨其配伍规律。方法:将四妙勇安汤拆方分为8组,采用HPLC测定哈巴苷含量,色谱条件为SunFireTMC18色谱柱(4.6 mm×150 mm,5μm),流动相0.1%磷酸水-乙腈梯度洗脱,流速1.0 mL.min-1,检测波长250 nm,柱温35℃,分析哈巴苷的提取率变化。结果:哈巴苷回归方程Y=1.22×106X+3 218.75(r=0.999 8),线性范围0.065 3～0.457μg,平均回收率96.81%(RSD 1.83%);哈巴苷提取率顺序为全方>玄参>拆方。结论:四妙勇安汤不同拆方均使哈巴苷的提取率降低,显示出全方合煎的优势,哈巴苷可能是四妙勇安汤发挥药效的有效组分之一。Objective： To investigate extraction rate variation of harpagide in different separated recipes of Simiao Yong＇an decoction,and explore its regularity of compatibility.Method： Extraction rate of harpagide from 8 separated recipes of Simiao Yong＇an decoction was determined by HPLC,using a SunFireTM C18 column（4.6 mm ×150 mm,5 μm）,acetonitrile-water containing 0.1% H3PO4 in gradient elution as mobile phase,the detection wavelength was set at 250 nm,the flow rate was 1.0 mL.min-1,column temperature of 35 ℃,then extraction rate variation of harpagide was analyzed.Result： The regression equation of harpagide was Y = 1.22 × 106X ＋ 3 218.75（r = 0.999 8） with the liner range of 0.065 3-0.457 μg,the average recovery was 96.81% with RSD of 1.83%.Extraction rate of harpagide from different separated recipes was in order of the original prescription Scrophularia ningpoensis other separated recipes.Conclusion： All these seven separated recipes of Simiao Yong＇an decoction reduced extraction rate of harpagide,which showed a distinct advantage of the original prescription.And harpagide may be one of effective components in Simiao Yong＇an decoction.

关 键 词：四妙勇安汤 拆方 配伍 哈巴苷 提取率 

分 类 号：R283.6[医药卫生—中药学]