HPLC-ELSD测定地黄不同炮制品中单糖含量  

Determination of Monosaccharide Content in Diferent Processed Radix Rehmannia Praeparata by HPLC-ELSD

作　　者：胡志方[1] 王小平[1] 陈建章[1] 

机构地区：[1]江西中医药高等专科学校,江西抚州344000

出　　处：《中国实验方剂学杂志》2013年第13期72-74,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81160520);江西省自然科学基金项目(2009GZY0114)

摘　　要：目的:建立HPLC-ELSD测定地黄不同炮制品中单糖含量方法,研究不同炮制方法对熟地黄单糖含量的影响。方法:采用高效液相色谱法,以蒸发光散射检测器进行检测,Prevail carbohydrate ES色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-水(75∶25),流速0.8 mL.min-1,柱温30℃,ELSD条件漂移管温度65℃,空气流速2.5 L.min-1,增益1。结果:D-葡萄糖平均回收率为98.24%,RSD 2.18%;D-果糖平均回收率为98.37%,RSD 2.11%;生地、炆制地黄、清蒸地黄、酒炖地黄中单糖含量分别为1.62%,(27.22±2.74)%,(9.63±2.05)%,(16.97±2.62)%。结论:该方法操作简便,可靠稳定,结果准确;地黄炮制品中单糖含量远远高于生地黄,炆制地黄中单糖含量高于其他两种炮制方法,为阐明其炮制机理提供实验依据。Objective： To establish an HPLC-ELSD method for determination of monosaccharide content in different processed Radix Rehmannia Praeparata and to study the effect of different processed method on monosaccharide content in Radix Rehmannia Praeparata.Method： HPLC-ELSD was applied to analyze the content of monosaccharide with prevail carbohydrate ES column（4.6 mm × 250 mm,5 μm）,acetonitrile-water（75∶ 25） as mobile phase.The flow rate was 0.8 mL.min-1;column temperature was kept at 30 ℃;temperature of drift tube set at 65 ℃,gas flow 2.5 L.min-1and gain 1.Result： The D-glucose and D-fructose average recovery was 98.24%（RSD 2.18%） and 98.37%（RSD 2.11%） respectively.The content of monosaccharide in Radix Rehmanniae from Wenzhi,steaming and stewing by liqour were 1.62%,27.22% ± 2.74%,9.63% ± 2.05%,16.97% ± 2.62% respectively.Conclusion： The method is simple,accurate and reproducible.The content of monosaccharide in Wenzhi Dihuang was higher than that of steaming Dihuang and stewing by liqour.It provides an experimental basis to illustrate its processing mechanisms.

关 键 词：高效液相色谱-蒸发光散射检测法 熟地黄 炆制法 D-葡萄糖 D-果糖 

分 类 号：R284.1[医药卫生—中药学]