UPLC-DAD法研究栽培两面针不同生长期不同部位有效成分的动态累积  

Dynamic Accumulation Research of Active Ingredient in Different Part and Growth Phase of Caltivative Zanthoxylum nitidum by UPLC-DAD

作　　者：何丽丽[1] 林钻煌[2] 胡永志[2] 覃青云[2] 黄光伟[2] 胡东南[1] 

机构地区：[1]广西壮族自治区药用植物园,南宁530023 [2]广西柳州两面针股份有限公司,广西柳州545001

出　　处：《中国实验方剂学杂志》2013年第13期165-168,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西科学研究及技术开发计划项目(桂科攻0815005-2-11)

摘　　要：目的:研究栽培两面针有效成分在不同生长期及不同药用部位的动态累积规律,以期为两面针合理用药及人工优质栽培提供理论依据。方法:采用UPLC-DAD法,测定栽培两面针药材不同生长期、不同药用部位(根、茎、枝)中氯化两面针碱含量变化。结果:两面针根、茎、枝不同部位中均含有氯化两面针碱,但含量差异较大,依次为根>茎>枝条;不同部位、不同生长期两面针药材中氯化两面针碱含量呈规律变化,出现两个较明显的高峰及一个低谷,即5,10～11月含量较高,6月含量最低。结论:两面针不同生长期,不同部位有效成分累积分布不同,根据其含量变化规律,建议两面针最佳采收期在栽培第3年的10～11月,药用部位为根部。两面针全株植物均具有一定的药用价值。Objective： To evaluate dynamic accumulation of active ingredient in different part and growth phase of caltivative Zanthoxylum nitidum,and provide the basis for the GAP planting and the rational utilization.Method： A sensitive and accurate method was developed to ditermined the nitidine chloride in different parts（root,stem,and branch） and different harvest times of Z.nitidum by ultra performance liquid chromatography（UPLC） with diode array detector（DAD） for the first time.Result： The variation of the nitidine chloride contents was existed in different parts of Z.nitidum,which sequence was showed as follows： root stem branch,there was regulation dynamic changes of nitidine chloride content in different part and growth phase,which exist two obvious peak and a valley,that is the content of nitidine chloride reached highest value at May and October and lowest at June.Conclusion： The active ingredient distributed in caltivative Z.nitidum was different in different part and growth phase,the optimal harvest time was at October and November in the third-year,and the root was the main medicinal parts.All part of Z.nitidum have the medicinal value.

关 键 词：栽培两面针 氯化两面针碱 动态累积 UPLC-DAD 含量 最佳采收期 

分 类 号：R282.2[医药卫生—中药学]