复方脑忆源片主要成分淫羊藿苷在Caco-2细胞模型上的转运研究  

Membrane Transport of the Icariin,which is Main Component of Naoyiyuan Tables Across the Caco-2 Monolayer

作　　者：周改莲[1] 单桂芝[2] 林励[3] 王莲婧[3] 

机构地区：[1]广西中医药大学药学院,南宁530001 [2]河南省水利水电学校,河南周口466001 [3]广州中医药大学中药学院,广州510006

出　　处：《中国实验方剂学杂志》2013年第13期178-181,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：海南省重点科技计划项目(090603)

摘　　要：目的:对复方脑忆源片中主要成分淫羊藿苷进行转运研究,阐明吸收特点。方法:利用Caco-2细胞模型,对复方脑忆源片中主要成分淫羊藿苷(ICA)进行体外转运,并通过高效液相(Luna Kromasil C18色谱柱(4.6 mm×150 mm,5μm);流动相乙腈∶水(30∶70);流速1.0 mL.min-1;检测波长270 nm;)测定单体、药材及复方中的淫羊藿苷转运后的含量。结果:淫羊藿苷在小肠吸收转运过程随时间增长而增加,可能会被Caco-2细胞中的P-gp转运蛋白外排。与淫羊藿单体和淫羊藿提取物中的淫羊藿苷比较,脑忆源片可显著提高淫羊藿苷的转运,同时可降低淫羊藿苷的外排作用。结论:在脑忆源片中,由于淫羊藿苷外排减少,可认为通过复方配伍后,方中的其他药物抑制了淫羊藿苷的外排,使其可以更好的发挥药理作用。Objective： The absorption characteristic of the icariin（ICA）,which is the main component of Naoyiyuan tables,was clarified by the membrane transport study.Method： The Caco-2 monolayer was used to study the transport of the ICA in monomer,herbs,and compounds in vitro.For the determination of the content of ICA,the HPLC method was operated with acetonitrile-water（30∶ 70） as mobile phase,with Luna Kromasil C18 column.The flow rate was 1.0 mL.min-1,detection wavelength was at 270 nm.Result： ICA exhibited timedependent permeation with apparent permeability coefficient P-gp values in the range 1 × 10-5-1 × 10-6cm.s-1,suggesting transcellular diffusion pathways.P-glycoprotein may play a role in the intestinal efflux of icariin.However compared with the icariin in monomer and herbs,the transport of ICA in Naoyiyuan tables is higher.Conclusion： The intestinal efflux of ICA in Naoyiyuan tables was reduced.So we can considered that the other ingredients in Naoyiyuan tables inhibited the efflux of icariin.It is profit for the pharmacological actions.

关 键 词：脑忆源片 CACO-2细胞模型 转运 淫羊藿苷 

分 类 号：R285.5[医药卫生—中药学]