麻黄加术汤对呼吸道合胞病毒感染小鼠血清IL-2,IFN-γ的影响  

Effect of Mahuang Jiazhu Decoction on Serum IL-2,IFN-γ Content in Mice Infected by RSV in the Damp and Cold Environment

作　　者：李俊莲[1] 李艳彦[1] 高鹏[2] 郭彩云[2] 郭晓峰[1] 马彦平[1] 陶功定[1] 

机构地区：[1]山西中医学院,太原030024 [2]长春中医药大学,吉林130117

出　　处：《中国实验方剂学杂志》2013年第13期196-199,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30873211);山西省国际合作项目(2011081065);山西省自然科学基金(200911049-5)

摘　　要：目的:探讨麻黄加术汤对寒湿环境因素下的呼吸道合胞病毒(RSV)感染小鼠血清白介素-2(IL-2)、干扰素-γ(IFN-γ)含量的影响以及在生态医学思想下寒湿环境、中西药物与机体免疫状态之间的相关性研究。方法:借助人工气候箱,模拟寒湿外邪环境,采用呼吸道合胞病毒0.1 mL滴鼻感染,麻黄加术汤0.472 5 g.mL-1、利巴韦林5.625 g.L-1灌胃,通过ELISA法检测血清IL-2,IFN-γ的含量。结果:与正常组相比,病毒模型组和寒湿组均使小鼠IL-2,IFN-γ含量显著下降(均P<0.001),寒湿外邪与病毒感染对小鼠IL-2,IFN-γ含量的影响有交互作用(P<0.001和P<0.05)。与病毒模型组和寒湿+病毒模型组分别相比,麻黄加术汤和利巴韦林均使小鼠IL-2,IFN-γ含量显著升高(P<0.001),而IL-2含量在中、西药组间无显著性差异,但IFN-γ含量的提高,在病毒+麻黄加术汤组显著低于病毒+利巴韦林组(P<0.001);与病毒+麻黄加术汤组比较,寒湿+病毒+麻黄加术汤组IFN-γ含量显著降低(P<0.01)。结论:寒湿外邪与病毒感染均可以使小鼠免疫力下降,西药抗病毒、中药散寒祛湿的麻黄加术汤均可以改善寒湿环境下病毒感染小鼠免疫状态。Objective： To study the effect of Mahuang Jiazhu decoction on the serum interleukin-2（IL-2） and interferon-γ（IFN-γ） content of mice infected by RSV in the damp and cold environment,and the correlation of immune state of mice with damp and cold environment and chinese and western drugs under ecological medicine.Method： The serum IL-2 and IFN-γ contents were examined with ELISA in mice which were placed artificial climate box to mimic the environment of simulating cold-wet exopathogen,to analyze the effects of Mahuang Jiazhu decoction and cold-wet exopathogen on immune state of mice infected by RSV.Result： The IL-2 and IFN-γ contents in virus model group and cold-wet group were lower significantly（all P 0.001）（all P 0.001） than that in normal group.There was difference in IL-2,IFN-γ contents between virus model group and cold-wet groups（P 0.001,P〈0.05）.The IL-2 and IFN-γ contents in the Mahuang Jiazhu decoction group and Ribavirin group were higher significantly than that in virus model group and cold-wet plus virus model groups,respectively（P 0.001）.But there was no difference in the IL-2 content between Mahuang Jiazhu decoction group and Ribavirin group.The IFN-γ contents in virus plus Mahuang Jiazhu decoction group was lower than that in virus plus Ribavirin group（P 0.001）.The IFN-γ contents in cold-wet plus virus plus Mahuang Jiazhu decoction group was lower than that in virus plus Mahuang Jiazhu decoction group（P〈0.01）.Conclusion： The decreased immunity of mice can be caused cold-wet exopathogen and virus infection.Mahuang Jiazhu decoction and Ribavirin can improve the immune state of mice infected by RSV in the damp and cold environment.

关 键 词：人工气候箱 上呼吸道感染 寒湿邪气 白介素-2 干扰素-γ 生态病因 

分 类 号：R285.5[医药卫生—中药学]